Evaluation of the Effect of Chinese Medicine Formula Kai-Xin-San Combined with Fluoxetine on Hippocampal Neural Stem Cells in Chronic Stress Induced Depression Model Mice
- VernacularTitle:中药复方开心散联用氟西汀对慢性压力应激抑郁模型小鼠海马神经干细胞的影响评价研究
- Author:
Lingxin HUANG
1
;
Xin LI
;
Lei YUAN
;
Yun ZHU
;
Xiaoning HUANG
;
Xuan LI
;
Huaqiang ZHAN
;
Jinao DUAN
;
Lejun LI
;
Yue ZHU
Author Information
- Publication Type:Journal Article
- Keywords: Kai-Xin-San; Fluoxetine; Chronic mild stress depression model; Combination medications; Hippocampal neural stem cells
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(4):1035-1046
- CountryChina
- Language:Chinese
- Abstract: Objective To evaluate the effect of Kaixin San(KXS)combined with fluoxetine on hippocampal neural stem cells in mice with chronic stress stress and depression.Methods A mouse model of depression was constructed using the method of chronic unpredictable stress stress,and the highest dose of KXS water extract and fluoxetine for clinical application was given for 28 days,and behavioral tests were carried out.Nissl staining was used to detect the pathological status of hippocampal tissues in mice.The expression of TUNEL and Nestin in mouse hippocampus was determined by immunofluorescence.Western blotting was used to detect the expressions of apoptotic proteins cleaved caspase-3 and caspase-3,pyroptosis proteins GSDMD and cleaved caspase-1,as well as the expression of neural stem cell marker Nestin in the hippocampus,and the expression of Wnt/β-catenin signaling pathway-related proteins in the hippocampus.Results The combination of KXS extract and fluoxetine can significantly improve the depression-like behavior of model mice,and the effect is better than fluoxetine alone.The combination inhibited the activation of apoptosis and pyroptosis signaling pathways in the hippocampus when used alone with high-dose fluoxetine,significantly upregulated the expression of Nestin,and regulated the expression of Wnt/β-catenin signaling pathway protein.Conclusion The combination of KXS and high-dose fluoxetine can improve apoptosis and pyroptosis in the hippocampus of stress stress and depression model mice,and upregulate the expression of neural stem cell marker Nestin by regulating the Wnt/β-catenin signaling pathway,which may be a key link to improve the antidepressant effect of the combination drug.
