Survey of genetic diversity of select tick species in Inner Mongolia
10.3969/j.issn.1002-2694.2025.00.028
- VernacularTitle:内蒙古地区三种蜱的遗传多样性研究
- Author:
Meng-yu CUI
1
;
Si SU
;
Lan MU
;
Rui-juan GAO
;
Qi-qi GUO
;
Hong REN
;
Li-li BAO
;
Jing-feng YU
Author Information
1. 安阳市肿瘤医院公共卫生科,安阳 455000;河南科技大学附属安阳市肿瘤医院公共卫生科,安阳 455000
- Publication Type:Journal Article
- Keywords:
Hyalomma marginatum;
Haemaphysalis concinna;
Argas persicus;
sequence characteristics;
genetic diversity
- From:
Chinese Journal of Zoonoses
2025;41(2):171-177
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to understand the internal genetic diversity and population history dynamics of ticks in Inner Mongolia,to provide data for designing effective vector control programs and revealing ticks'transmission mechanisms.From 2022 to 2023,the manual collection method was used to collect samples in Inner Mongolia.The 16S rDNA and COI gene sequences of ticks were used to identify Hyalomma marginatum,Haemaphysalis concinna,and Argas persicus,and analyze the sequence characteristics and genetic diversity within the populations.Base composition analysis indicated that the average A+T content of the 16S rDNA gene and CO I gene in the three ticks was significantly higher than that of C+G.Moreover,22 haplotypes of the COI gene and 12 haplotypes of the 16S rDNA sequence were identified in Hyalomma marginatum.Eleven haplotypes were identified according to the COI gene,and nine haplotypes were identified according to the16S rDNA sequence of Haemaphysalis concinna.Two haplotypes were identified on the basis of the COI gene,and six haplotypes were identified on the basis of the 16S rDNA sequence of Ar gas persicus.The minimum 16S rDNA haplotype diversity was 0.264 for Ar gas persicus and 0.579 for the other two species.The nucleotide diversity of the three tick species was less than 0.05.Tajima's val-ue and Fu's Fs value of the neutrality test were negative.Base saturation substitution analysis indicated that neither of the two genes in the three tick species reached saturation.The phylogenetic tree revealed that Hyalomma marginatum,Haema physalis concinna,and Ar gas persicus in Inner Mongolia independently aggregated into branches.In conclusion,the base content of Hyalomma marginatum,Haemaphysalis concinna,and Argas persicus genes in Inner Mongolia was consist-ent with the characteristics of insect mitochondrial DNA content.Furthermore,the three tick populations showed rapid evolu-tionary population expansion,and the phylogeny of three tick species showed independent aggregation into clades,with no pop-ulation isolation.
