The diagnostic value and clinical research of 5'-tRF-GlyGCC in the early stage of colorectal cancer
10.3969/j.issn.1006-5725.2025.11.018
- VernacularTitle:5'-tRF-GlyGCC在结直肠癌早期诊断价值
- Author:
Long WANG
1
;
Yingmin WU
;
Sha SHANG
;
Lu WANG
Author Information
1. 贵州医科大学 基础医学院生理学教研室(贵州贵阳 561113)
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
peripheral blood;
tRNA-derived small RNA fragments;
early diagnosis
- From:
The Journal of Practical Medicine
2025;41(11):1730-1735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the content of 5'-tRF-GlyGCC in peripheral blood and explore its applica-tion value in the early diagnosis of colorectal cancer(CRC).Methods Peripheral blood samples were randomly collected from 99 CRC patients,11 patients with enteritis,17 patients with colorectal polyps and 99 healthy indi-viduals in the same period at the Affiliated Hospital of Guizhou Medical University.The content of 5'-tRF-GlyGCC in total RNA of peripheral blood was detected by fluorescence RT-PCR.The receiver operating characteristic curve(ROC)was established to analyze the value of 5'-tRF-GlyGCC content in the early diagnosis of CRC and to explore the relationship between its content and the clinical and pathological characteristics of CRC patients.Results The content of 5'-tRF-GlyGCC in the peripheral blood of CRC patients and colorectal polyp patients was significantly higher than that of healthy individuals and patients with enteritis,and the differences were statistically significant(P<0.05).The area under the curve(AUC)of 5'-tRF-GlyGCC for diagnosing CRC was 0.817(95%CI:0.763～0.871),with a diagnostic sensitivity of 56.7%and a specificity of 99.9%.The content of 5'-tRF-GlyGCC in peripheral blood was significantly positively correlated with the levels of CEA and CA19-9 in CRC patients.Conclusion The detection of 5'-tRF-GlyGCC in peripheral blood has a high application value in the early diagnosis of CRC and can be used for clinical reference and research.
