Predictive Value of Serum miR-150 and miR-155 Expression Levels in Patients with Lower Limb Arteriosclerosis Obliterans for Restenosis after Stent Implantation
10.3969/j.issn.1671-7414.2025.02.020
- VernacularTitle:下肢动脉硬化闭塞症患者血清miR-150和miR-155水平表达对支架植入术再狭窄的预测价值
- Author:
Zhongjun ZHANG
1
;
Lin CHEN
Author Information
1. 德阳市人民医院介入科,四川 德阳 618000
- Publication Type:Journal Article
- Keywords:
lower limb arteriosclerosis obliterans;
in-stent restenosis;
micro RNA-150;
micro RNA-155
- From:
Journal of Modern Laboratory Medicine
2025;40(2):109-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the predictive value of serum microRNA(miR)-150 and microRNA(miR)-155 expression levels in patients with lower limb arteriosclerosis obliterans(ASO)for in-stent restenosis(ISR)after stent implantation.Methods A total of 108 ASO patients who received stent implantation in Deyang People's Hospital form March 2020 to June 2022 were gathered.They were grouped into two groups based on whether they had experienced ISR one year after surgery:the ISR group(n=42)and the non-ISR group(n=66).Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was applied to detect the relative expression levels of miR-150 and miR-155 in serum.Pearson correlation was applied to analyze the correlation between serum miR-150,miR-155 levels and low-density lipoprotein cholesterol(LDL-C)and hypersensitive C-reactive protein(hs-CRP).Multivariate logistic regression was applied to analyze the factors influencing the occurrence of ISR in ASO patients.The receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum miR-150 and miR-155 levels for ISR in ASO patients.Results Compared with non-ISR group,the serum miR-150(0.65±0.16 vs 1.01±0.09)level in ISR group was greatly reduced,while the levels of miR-155(1.68±0.39 vs 1.02±0.11),LDL-C(2.85±0.76 mmol/L vs 2.21±0.62mmol/L)and hs-CRP(2.36±0.47 mg/L vs 1.82±0.36mg/L)were greatly increased,and the differences were statistically significant(t=4.785～14.957,all P<0.05).According to Pearson correlation analysis,serum miR-150 was greatly negatively correlated with LDL-C and hs-CRP levels(r=-0.465,-0.532,all P<0.05),the serum miR-155 level was greatly positively correlated with LDL-C and hs-CRP levels(r=0.453,0.481,all P<0.05).Multivariate Logistic regression analysis showed that serum miR-150 was a protective factor affecting the occurrence of ISR in ASO patients,while serum miR-155,LDL-C and hs-CRP were risk factors affecting the occurrence of ISR in ASO patients(all P<0.05).ROC curve showed that the AUC(95%CI)of serum miR-150,miR-155,and their combined prediction for ISR in ASO patients was 0.869(0.791～0.926),0.874(0.796～0.930)and 0.976(0.926～0.996),respectively,the combined detection was greatly better than the two individual predictions(Z=2.679,2.745,all P<0.05).Conclusion The miR-150 level decreased and the miR-155 level increased in patients with ISR after ASO stent implantation are both factors affecting the occurrence of ISR in ASO patients,and the combination detection of the two has high predictive value.
