Function of Tim-3/Galectin-9 pathway and MDSC in aGVHD after Haplo-HDPSCT
10.3969/j.issn.1000-484X.2025.06.034
- VernacularTitle:Tim-3/Galectin-9通路和MDSC在亲缘间Haplo-HDPSCT后aGVHD中的作用
- Author:
Le ZHANG
1
;
Nannan PANG
;
Mingkai YU
;
Hailong YUAN
;
Ming JIANG
Author Information
1. 新疆医科大学第一临床医学院,乌鲁木齐 830054
- Publication Type:Journal Article
- Keywords:
Haplo-HDPSCT;
Acute graft-versus-host disease;
MDSC;
Tim-3/Galectin-9 signaling pathway;
CD8+T cells
- From:
Chinese Journal of Immunology
2025;41(6):1467-1472
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the possible effects of Tim-3/Galectin-9 signaling pathway and MDSC on acute graft-ver-sus-host disease(aGVHD)development in patients after Haplo-HDPSCT.Methods:A total of 42 patients underwent Haplo-HDPSCT and 20 healthy controls were enrolled in this study.Peripheral blood specimens were collected from all study subjects,the number of Tim-3+CD8+T,Granzyme B+CD8+T and MDSC were detected by FCM,and level of Galectin-9 was detected by ELISA.Apoptosis of CD8+T cells from peripheral blood of aGVHD patients were detected by FCM.Results:According to the Seattle International Diagnostic Criteria,there were 16 patients occurred aGVHD after transplantation,while 26 patients did not.①The number of Tim-3+CD8+T and Granzyme B+CD8+T cells in aGVHD patients were significantly higher than patients without aGVHD and healthy controls,while MDSC and Galectin-9 in aGVHD patients were lower than patients without aGVHD and healthy controls(P<0.05);②There were statistically significant differences in the number of Tim-3+CD8+T and Granzyme B+CD8+T cells and level of Galectin-9 between patients with mild(grade Ⅰ～Ⅱ)and severe(grade Ⅲ～Ⅳ)aGVHD groups(P<0.05),while there was no statistically significant difference in the number of MDSC(P=0.689);③Spearman analysis showed that level of Galectin-9 was positively correlated with the number of MDSC in patients without aGVHD(r=0.684,P<0.05);④Isolating CD8+T cells from peripheral blood of aGVHD patients,and found that addition of Galectin-9 increased rate of CD8+T cells apoptosis.Conclusion:Galectin-9 inhibits immune response of CD8+T cells through the Tim-3/Galectin-9 signaling pathway after Haplo-HDPSCT,and this can avoid or slow down the incidence of aGVHD;Galectin-9 is positively correlated with the number of MDSC,it may be associated with the occurrence of aGVHD.
