Effect of paeoniflorin on myocardial injury in rats with coronary atherosclerotic heart disease by regulating SDF-1/CXCR4 signaling pathway
10.3969/j.issn.1000-484X.2025.06.032
- VernacularTitle:芍药苷调节SDF-1/CXCR4信号通路对冠心病大鼠心肌损伤的影响
- Author:
Wenqian WANG
1
;
Jie WANG
Author Information
1. 湖北中医药大学中医临床学院,武汉市中医医院,武汉 430065
- Publication Type:Journal Article
- Keywords:
Paeoniflorin;
Stromal cell-derived factor-1;
CXC chemokine receptor 4;
Coronary atherosclerotic heart disease;
Myocardial injury
- From:
Chinese Journal of Immunology
2025;41(6):1455-1460
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of paeoniflorin(PF)on myocardial injury in rats with coronary atherosclerotic heart disease(CHD)by regulating stromal cell-derived factor-1(SDF-1)/CXC chemokine receptor 4(CXCR4)signaling pathway.Methods:A CHD rat model was established using a high-fat diet combined with intraperitoneal injection of pituitrin.The successfully established model rats were randomly separated into PF low,medium,and high doses groups,activator group and Model group,according to the random number table method.Another 18 rats were randomly selected as control group.Corresponding drugs were administered by gavage or intraperitoneal injection once a day for 4 consecutive weeks.HE and TUNEL staining were applied to observe myocardial pathological damage and apoptosis of rats;ELISA was applied to detect levels of serum myocardial injury indicators,includ-ing myoglobin(Mb),creatine kinase isoenzyme(CK-MB),creatine kinase(CK),TNF-α,IL-1β and IL-6;automatic biochemical analyzer was applied to measure serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)of rats;kits were applied to detect levels of malondialdehyde(MDA),reactive oxygen spe-cies(ROS)and Glutathione peroxidase(GSH-Px)in rats myocardial tissues;Western blot was applied to detect expressions of cleaved-cysteinyl aspartate specific proteinase 3(cleaved-caspase-3)and SDF-1/CXCR4 pathway proteins in myocardial tissue.Results:Compared with control group,myocardial cell apoptosis rate,Mb,CK-MB,CK,TNF-α,IL-1β,IL-6,TC,TG,LDL-C,MDA,ROS,cleaved-caspase-3,SDF-1,CXCR4 protein levels in Model group were obviously increased,while levels of HDL-C and GSH-Px were obviously reduced(P<0.05);compared with Model group,apoptosis rate of myocardial cells,Mb,CK-MB,CK,TNF-α,IL-1β,IL-6,TC,TG,LDL-C,MDA,ROS,cleaved-caspase-3,SDF-1,CXCR4 protein levels in low,medium and high doses PF groups were obviously reduced,while levels of HDL-C and GSH-Px were obviously increased(P<0.05);compared with high-dose PF group,changes in the above indicators in activator group of rats were obviously reversed(P<0.05).Conclusion:PF may alleviate myo-cardial injury in CHD rats by inhibiting the SDF-1/CXCR4 signaling pathway,inhibiting inflammation,oxidative stress and cell apop-tosis.
