Mechanism of effect of baicalin on macrophage polarization in rats with SAP lung injury based on JAK2/STAT3 signaling pathway
10.3969/j.issn.1000-484X.2025.06.025
- VernacularTitle:基于JAK2/STAT3信号观察黄芩苷对SAP肺损伤大鼠巨噬细胞极化的影响及机制
- Author:
Xiaobo SHUI
1
;
Shaojiang ZHANG
1
Author Information
1. 遵义市第一人民医院,遵义医科大学第三附属医院,遵义 563000
- Publication Type:Journal Article
- Keywords:
Baicalin;
Severe acute pancreatitis;
Lung injury;
Macrophages polarization;
Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway
- From:
Chinese Journal of Immunology
2025;41(6):1407-1414
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of baicalin(BA)on the polarization of macrophages in rats with lung injury in-duced by severe acute pancreatitis(SAP),and its regulatory mechanism on janus protein tyrosine kinase 2/signal transducer and acti-vator of transcription 3(JAK2/STAT3)signaling pathway.Methods:The SAP rat model was constructed.Low and high doses of BA were used for intervention.SC-39100,a specific agonist of JAK2/STAT3,was used to rescue the function.ELISA was used to detect the content of TNF-α,IL-6 and IL-10.TUNEL was used to detect apoptosis in lung tissue.Immunofluorescence was used to detect the polarization of macrophages in lung tissue.Western blot was used to detect cell the expression of phosphorylated protein tyrosine ki-nase 2(p-JAK2),phosphorylated signal transducer and activator of transcription 3(p-STAT3),TNF-α,IL-6,IL-10,TGF-β1 in lung tissue.Results:BA could significantly downregulate the content of TNF-α and IL-6 in serum,as well as the rate of cell apoptosis in lung tissue.BA could significantly upregulate the content of IL-10 in serum.BA could regulate the polarization of macrophages to-wards M2.Western blot results showed that BA could significantly downregulate the expressions of p-JAK2,p-STAT3,TNF-α and IL-6,upregulate the expressions of IL-10 and TGF-β1.The expression of SC-39100 could partially reverse the protective effect of BA on lung tissue,and the differences were statistically significant(all P<0.05).Conclusion:BA can significantly inhibit cell apoptosis in the rat model of SAP lung injury,alleviate the pathological damage of the animal lung tissue,and promote the polarization of macro-phages in the lung tissue towards M2,which may be related to the inhibition of JAK2/STAT3 signaling pathway phosphorylation.
