Role of TLR4/ERK1/2 signaling pathway in progesterone regulating IL-8 expression in decidual stromal cells
10.3969/j.issn.1000-484X.2025.06.021
- VernacularTitle:TLR4/ERK1/2信号通路在孕酮调节蜕膜基质细胞IL-8表达中的作用
- Author:
Ziwei ZHAO
1
;
Na LI
;
Meixia YANG
;
Xiaoling GONG
;
Zhaojin LUAN
Author Information
1. 包头医学院组织学与胚胎学教研室,包头 014000
- Publication Type:Journal Article
- Keywords:
TLR4/ERK1/2 signaling pathway;
Progesterone;
Decidual stromal cells;
IL-8
- From:
Chinese Journal of Immunology
2025;41(6):1383-1387
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of TLR4/ERK1/2 signaling pathway in progesterone regulating expression of IL-8 in decidual stromal cells(DSCs).Methods:Human DSCs on early pregnancy were isolated and cultured in vitro.The cells were treated with 0.01 μmol/L,0.1 μmol/L and 1 μmol/L progesterone,respectively.Expressions of IL-8,TLR4,ERK1/2 and p-ERK1/2 were detected by Western blot.TLR4 inhibitor TAK-242 and ERK1/2 inhibitor U0126 were further treated,and expression of IL-8 in progesteron+inhibitor group,progesterone group and control group was detected by Western blot.Results:With the increase of pro-gesterone concentration,expression of IL-8 in DSCs decreased gradually.Expressions of DSCs IL-8,TLR4 and p-ERK1/2 in 0.01 μmol/L progesterone group were lower than those in control group(P=0.035 8,P=0.019 4 and P=0.047 1).Expressions of IL-8,TLR4 and p-ERK1/2 in DSCs in 0.1 μmol/L progesterone group were significantly lower than those in control group(P=0.003 5,P=0.004 0 and P=0.009 9).Expressions of IL-8,TLR4 and p-ERK1/2 in DSCs in 1 μmol/L progesterone group were significantly lower than those in control group(P=0.003 3,P=0.001 6 and P=0.005 0).Expression of IL-8 in progesterone+TAK-242 group was signifi-cantly lower than that in progesterone group(P=0.009 1),and expression of IL-8 in progesterone+U0126 group was significantly lower than that in progesterone group(P=0.004).Conclusion:TLR4/ERK1/2 signaling pathway is involved in the physiological process of progesterone regulating expression of IL-8 in DSCs,and progesterone reduces IL-8 expression by inhibiting expression of TLR4 and phosphorylation activation of ERK1/2.
