Preliminary investigation on the diagnostic values of sPD-1 and sPD-L1 changes in the acute rejection of rats after liver transplantation
10.3760/cma.j.cn421203-20240816-00180
- VernacularTitle:sPD-1、sPD-L1水平变化在大鼠肝移植后急性排斥反应中诊断价值的初步探讨
- Author:
Jiaowen YANG
1
;
Tao CHEN
;
Zhenglu WANG
;
Lei CAO
;
Jinpeng TU
;
Hong ZHENG
;
Wei GAO
;
Zhongyang SHEN
Author Information
1. 天津医科大学第一中心临床医学院，天津 300190
- Publication Type:Journal Article
- Keywords:
Rats;
Liver transplantation;
Acute rejection;
Soluble programmed cell death protein 1;
Soluble programmed cell death ligand 1
- From:
Chinese Journal of Organ Transplantation
2025;46(3):226-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential diagnostic value of peripheral blood soluble programmed cell death protein 1 (sPD-1) and soluble programmed cell death protein ligand 1 (sPD-L1) in acute rejection (AR) following liver transplantation using a rat model.Methods:A rat liver transplantation AR model was established, with the AR group (Lewis→BN) set as the experimental group (n=6) and the non-AR group (BN→BN) as the control group (n=6). Peripheral blood sPD-1 and sPD-L1 concentrations were measured using enzyme-linked immunosorbent assay (ELISA) at 1 day before transplantation and at 1, 3, and 7 days postoperatively. On postoperative day 7, the expression levels of PD-1 and PD-L1 in liver tissues were detected by immunohistochemistry (IHC). Independent samples t-test and repeated measures ANOVA were used to compare the results between the two groups. Pearson correlation analysis was conducted to evaluate the relationship between sPD-1, sPD-L1, the sPD-1/sPD-L1 ratio, and the rejection activity index.Results:On postoperative day 7, the experimental group exhibited significantly higher peripheral sPD-1 levels (218.59±36.88 vs. 164.95±15.82 ng/L) and a higher sPD-1/sPD-L1 ratio (0.44±0.12 vs. 0.36±0.07), but lower sPD-L1 levels (379.56±73.41 vs. 423.64±96.55 ng/L) compared to the control group (all P<0.05). Correlation analysis revealed a significant positive correlation between sPD-1 levels and the rejection activity index ( r=0.680, P<0.05), as well as between the sPD-1/sPD-L1 ratio and the rejection activity index ( r=0.795, P<0.01), while no correlation was observed between sPD-L1 levels and the rejection activity index. IHC demonstrated positive PD-1 and PD-L1 expression in the liver tissues of the experimental group, whereas the control group showed negative expression. Conclusion:Peripheral blood sPD-1 levels and the sPD-1/sPD-L1 ratio are significantly associated with AR after liver transplantation in rats, suggesting their potential as biomarkers for diagnosing AR in liver transplant recipients.
