ERMAP ameliorates experimental autoimmune encephalomyelitis in MOG-specific TCR transgenic mice
10.3969/j.issn.1000-484X.2025.06.015
- VernacularTitle:ERMAP改善MOG特异性TCR转基因小鼠实验性自身免疫性脑脊髓炎
- Author:
Jie ZHU
1
;
Wenqian SONG
;
Kezhu CHEN
;
Yuandi LI
;
Jie GAO
;
Rong HU
;
Min SU
Author Information
1. 贵州医科大学基础医学院组织学与胚胎学教研室,贵阳 550025
- Publication Type:Journal Article
- Keywords:
ERMAP;
Autoreactive T cells;
2D2TCR;
Experimental autoimmune encephalomyelitis
- From:
Chinese Journal of Immunology
2025;41(6):1344-1349
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To induce an experimental autoimmune encephalomyelitis(EAE)model by MOG-specific TCR trans-genic mice(2D2TCR transgenic mice),and to investigate effect of exogenous ERMAP on T cells in spleen of MOG35-55-induced 2D2TCR transgenic mice.Methods:EAE models were established in two groups of 2D2TCR transgenic mice(Control-Ig treatment for control group and ERMAP-Ig fusion protein treatment for experimental group),with 9 mice per group.Severity of spinal cord injury of MOG35-55-induced EAE in mice was assessed based on daily clinical scores(DAI),HE and LFB staining results;autoreactive T cells(CD4+Vα3.2+Vβ11+),T cell proliferation activation indicators CD69(CD4+Vα3.2+Vβ11+CD69+)and Ki67(CD4+Vα3.2+Vβ11+Ki67+),Treg(CD4+Vα3.2+Vβ11+CD25+Foxp3+)and Th17 cells(CD4+Vα3.2+Vβ11+IL-17A+)in spleen were detected by flow cytome-try;IL-17A,IL-6,IFN-γ and TGF-β expressions in spinal cord tissues were detected by qRT-PCR.Results:In MOG35-55-induced 2D2TCR transgenic mouse EAE model,ERMAP-Ig fusion protein treatment group showed milder inflammatory infiltration and demye-lination in spinal cord,decreased proportion of autoreactive T cells,decreased proportion of activated and proliferating T cells,increased proportion of Treg,inhibition of Th17 cell differentiation,less inflammatory cell aggregation and cytokine production,and increased expression of anti-inflammatory factors in spinal cord.Conclusion:ERMAP may be involved in development of EAE in 2D2TCR transgenic mice by inhibiting T cell proliferative activation and promoting Treg cell production.
