Mechanism of disidin domain receptor 1 regulating the proliferation and apoptosis of glioma cells
- VernacularTitle:盘状蛋白结构域受体1调控胶质瘤细胞增殖和凋亡的机制研究
- Author:
Kai-tao ZHAO
1
;
Jun-li LIANG
1
;
Rong-jun YANG
1
;
Dong HUANG
1
;
Rui MIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: glioma; disidin domain receptor 1; proliferation; apoptosis; PI3K/AKT pathway
- From: Journal of Regional Anatomy and Operative Surgery 2025;34(6):484-488
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the regulatory effect and mechanism of disidin domain receptor 1(DDR1)on the proliferation and apoptosis of glioma cells.Methods The expression levels of DDR1 in human glioma cells and normal glial cells were detected by qRT-PCR and Western blot.Glioma cells U251 with stable overexpression and knockdown of DDR1 were constructed by lentivirus infection,the proliferation ability of the stable cell line was detected by CCK-8 assay,and the apoptosis ability was detected by flow cytometry.Western blot was used to detect the activation of related pathways in stable cell lines and explore the regulatory mechanism of DDR1.Results The results of qRT-PCR and Western blot showed that the expression level of DDR1 in human glioma cell U251 was higher than those in human glioma cell U87 and normal glial cell HEB.Therefore,the U251 cell line was selected for subsequent experiments.CCK-8 assay showed that overexpression of DDR1 promote the proliferation of glioma cells,while knockdown of DDR1 inhibit the proliferation of glioma cells.The results of flow cytometry showed that overexpression of DDR1 inhibit the apoptosis of glioma cells,while knockdown of DDR1 induce the apoptosis of glioma cells.Western blot results showed that overexpression of DDR1 activate the PI3K/AKT pathway,while knockdown of DDR1 inhibit the activation of the PI3K/AKT pathway.Conclusion DDR1 can enhance the proliferation ability of glioma cells and inhibit their apoptosis by activating the PI3K/AKT pathway,promoting the development of glioma cells.Therefore,DDR1 may become a potential target for the treatment of glioma.
