Construction of miRNA-452/Let7i Ultrasound Molecular Probes Targeting hiPSCs-CMs
10.3969/j.issn.1005-5185.2025.06.008
- VernacularTitle:靶向hiPSCs-CMs的miRNA-452/Let7i超声分子探针构建
- Author:
Yibulayin MAIERHABA
1
;
Zihang WANG
;
Wei WANG
;
Lingjie YANG
;
Li'na GUAN
;
Yuming MU
Author Information
1. 新疆医科大学第一附属医院心脏超声诊断科,新疆 乌鲁木齐 830011;新疆超声医学重点实验室,新疆 乌鲁木齐 830011
- Publication Type:Journal Article
- Keywords:
Adenoviridae;
Transfection;
Ultrasonics;
Molecular probes;
Human induced pluripotent stem cell derived cardiomyocytes;
Experimental study
- From:
Chinese Journal of Medical Imaging
2025;33(6):611-617
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To construct an ultrasound molecular probe carrying miRNA-452/Let-7i and analyze its targeting effect on human induced pluripotent stem cell derived cardiomyocytes(hiPSCs-CMs)in vitro.Materials and Methods The biotinylated core lipid microbubbles(NBs),lipid microbubbles carrying adenovirus overexpressing miRNA-452/Let-7i(Ad-miR-452/Let-7i)and ultrasound molecular probes carrying adenovirus overexpressing miRNA-452/Let-7i and targeting protein MYH6(Ad-miR-452/Let7i-MYH6)were synthesized,and the expression rates of fluorescent proteins to verify their binding were compared.The physical and chemical properties,stability,and in vitro imaging of those abovementioned parameters were measured,respectively,and theirs biological safety was evaluated.hiPSCs-CMs was divided into Ad-miR-452/Let-7i group and Ad-miR-452/Let7i-MYH6 group,then theirs cell binding status were compared.Results The constructed NBs,Ad-miR-452/Let-7i and Ad-miR-452/Let7i-MYH6 had regular morphology and good stability.Detection data showed that theirs concentrations were(1.62±0.40)×108 particles/ml,(1.83±0.20)×108 particles/ml and(1.35±0.33)×108 particles/ml,respectively.The average particle sizes were(605.94±17.46)nm,(726.53±14.95)nm and(729.31±16.62)nm,respectively.The zeta potentials were(2.61±0.30)mV,(-11.21±0.71)mV and(-12.28±0.52)mV,respectively.The polydispersity indices were 0.24±0.07,0.26±0.14 and 0.25±0.13,respectively.With a concentration of 107 particles/ml,the imaging strengths of the three samples were 99.24±5.04,102.18±3.61 and 99.07±3.87,respectively.The miRNA-loaded ultrasound molecular probes exhibited no significant toxicity to the cells,with in vitro imaging lasting over 15 minutes,and they could specifically bind to hiPSC-CMs.Conclusion The constructed ultrasound molecular probe has stability and in vitro targeting,effectively improving virus transfection efficiency.
