Effect of CCL chemokine family on thyroid carcinoma and the construction of a risk model
- VernacularTitle:趋化因子CCL家族对甲状腺癌的影响及风险模型构建
- Author:
Yang MAO
1
;
Wen-jing WANG
1
;
De-feng LI
1
Author Information
- Publication Type:Journal Article
- Keywords: thyroid carcinoma; differentially expressed genes; CCL chemokine family; tumor immune microenvironment; risk model
- From: Journal of Regional Anatomy and Operative Surgery 2025;34(6):494-500
- CountryChina
- Language:Chinese
- Abstract: Objective By analyzing the gene expression data in TCGA-THCA dataset,the differentially expressed genes related to thyroid carcinoma(THCA)were identified,a risk model was construct,and the role of CCL chemokine family in the development of THCA and its effect on the tumor immune microenvironment were investigated.Methods TCGA-THCA dataset was downloaded and analyzed,including 512 cancer samples and 59 adjacent tissue samples.The differentially expressed genes were obtained by taking the intersection of R packages of differential analysis,and the principal component analysis(PCA),Gene Ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed.CellMarker,monocle,and CellChat were used to explore the Cell atlas,pseudotemporal trajectory,and cellular communication.Results A total of 698 up-regulated genes and 285 down-regulated genes were identified.PCA showed that there were significant differences in gene expression patterns between the THCA group and the normal group.GO and KEGG enrichment analysis revealed that differentially expressed genes were mainly involved in biological processes such as extracellular matrix tissue,cell junction assembly,and complement system.The results of risk model showed that the CCL genes was significantly correlated with poor prognosis(P=0.005).Single cell analysis revealed that fibroblasts played a key role in the development of THCA,and the expression of CCL gene was significantly up-regulated in fibroblasts(P<0.05).The results of pseudotemporal trajectory analysis showed that CCL gene was differentially expressed during cellular differentiation;the results of cellular communication analysis showed that the interaction between fibroblasts and endothelial cells is crucial in the tumor microenvironment.Conclusion The expression of CCL chemokine family in THCA is closely related to poor prognosis,especially CCL4,which activates the downstream signaling pathways by interacts with CCR receptors of cancer-associated fibroblasts,thereby promoting the development of cancer.These findings provide novel insights for understanding the role of CCL chemokine family in THCA and its regulation of the tumor immune microenvironment.
