Efficacy of the 45 mg 13C-urea breath test in the detection of Helicobacter pylori infection in adults
10.3760/cma.j.cn311367-20241029-00415
- VernacularTitle:45 mg 13C－尿素呼气试验检测成人幽门螺杆菌感染的有效性研究
- Author:
Qingjie ZHOU
1
;
Huang SU
1
;
Jie PAN
1
Author Information
1. 温州市中心医院消化内科，温州 325000
- Publication Type:Journal Article
- Keywords:
13C-urea breath test;
13C-urea breath analyzer;
45 mg 13C-urea tablet;
Helicobacter pylori
- From:
Chinese Journal of Digestion
2024;44(12):806-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the efficacy of the 45 mg 13C-urea breath test ( 13C-UBT) in the detection of Helicobacter pylori ( H. pylori) infection in adults. Methods:From November 1 to December 18 2021, a total of 255 patients who did 13C-UBT and gastroscopy examination in Wenzhou Central Hospital were recruited. The 13C-UBT was carried out with 45 mg 13C urea tablets by using BreathID, HY-50, and YH-08 H. pylori analyzers, respectively. During gastroscopy, 2 pieces of gastric mucosa tissues were taken from the adjacent part (< 1 cm) of gastric antrum and corpus for the urease test and histopathological examination (the gold standard), and the results were compared with those of 13C-UBT tests. The area under the curve (AUC) of the receiver operating characteristic curve (ROC) was used to evaluate the diagnostic efficacy of the three 13C-UBT analyzers for the detection of H. pylori infection. Kappa consistency test was used to evaluate the consistency of three detection methods. Results:A total of 237 subjects were included in this study. The accuracy, sensitivity, specificity, positive predictive value and negative predictive value of BreathID in diagnosing H. pylori infection were 95.34%, 92.91%, 98.96%, 0.992 and 0.905, respectively; the accuracy, sensitivity, specificity, positive predictive value and negative predictive value of HY-50 in diagnosing H. pylori infection were 94.51%, 90.78%, 100.00%, 1.000 and 0.881, respectively; the accuracy, sensitivity, specificity, positive predictive value and negative predictive value of HY-08 in diagnosing H. pylori infection were 91.98%, 91.49%, 92.71%, 0.948 and 0.881, respectively. The Kappa coefficients of the detection values of BreathID, HY-50, and YH-08 analyzers were 0.905, 0.889 and 0.835, respectively. The results of ROC showed that the Yoden indexes of BreathID, HY-50 and YH-08 analyzers were 0.919, 0.908 and 0.842, respectively, and the AUC values were 0.995, 0.993 and 0.969, respectively. Based on the Youden index, when using 45 mg 13C-urea tablets, the optimal preset cut-off values of BreathID, HY-50, and YH-08 with the best accuracy and authenticity in diagnosing H. pylori infection were 3.850, 3.965, and 3.350, respectively. Conclusions:The 45 mg 13C-UBT can achieve satisfactory detection accuracy and specificity for the detection of H. pylori infection in adults. It is recommended to set the cut-off value as an interval range, which can effectively improve the detection accuracy.
