PX-478 enhances the effect of lung cancer radiotherapy by regulating HIF-1α-mediated glycolysis
10.12092/j.issn.1009-2501.2025.07.008
- VernacularTitle:PX-478通过调节HIF-1α介导的糖酵解增强肺癌放疗效果的研究
- Author:
Gengqiang YANG
1
;
Yangyang LI
;
Qiyang LI
;
Shangzu ZHANG
;
Yue YANG
;
Ting ZHOU
;
Liying ZHANG
Author Information
1. 甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究重点实验室
- Publication Type:Journal Article
- Keywords:
PX-478;
radiotherapy;
glycolysis;
hy-poxia-inducible factor-1α
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(7):935-941
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the efficacy and molecu-lar mechanism of PX-478 in enhancing radiotherapy effect of lung cancer.METHODS:H460,A549 cells were divided into blank group,radiation group and radiation combined PX-478 group.In addition to the blank group,the radiation group and the PX-478 group were given 2Gy X-ray irradiation to estab-lish the radiation model,and the radiation com-bined with the PX-478 group was given 20 μmol/L PX-478 intervention after modeling,and cultured for 24 h.Inverted microscope was used to observe cell growth and cell number,CCK-8 method was used to detect cell viability,cloning was used to ob-serve cell proliferation,flow cytometry was used to detect cell apoptosis,and Western blot was used to detect HIF-1α,GLUT1,HK2,PFK1,PKM2,LDHA pro-tein expression.RESULTS:Compared with blank group,the number of H460,A549 cells in radiation group decreased,cell viability and proliferation abil-ity decreased,cell apoptosis rate increased,HIF-1α,GLUT1,HK2,PFK1,PKM2,LDHA protein expression increased(P＜0.01).Compared with the radiation group,the number of H460,A549 cells in the radia-tion combined PX-478 group was significantly de-creased,the cell viability and proliferation ability were significantly weakened,the apoptosis rate was significantly increased,and the protein expres-sions of HIF-1α,GLUT1,HK2,PFK1,PKM2 and LDHA were significantly decreased(P＜0.01).CONCLU-SION:PX-478 can regulate the HIF-1α-mediated gly-colysis in A549,H460 cells after radiation,regulate the energy metabolism,increase the apoptosis of tumor cells,and improve the effect of radiotherapy.
