Expression of sialylated CD15 in classical Hodgkin lymphoma following sialidase digestion
10.3760/cma.j.cn112151-20250528-00370
- VernacularTitle:唾液酸化的CD15在经典霍奇金淋巴瘤的表达
- Author:
Xiuli MA
1
;
Lixin ZHOU
;
Yunfei SHI
;
Yumei LAI
;
Xinting DIAO
;
Hongwei WANG
;
Xianghong LI
Author Information
1. 北京大学肿瘤医院暨北京市肿瘤防治研究所病理科 恶性肿瘤发病机制及转化研究教育部重点实验室，北京 100142
- Publication Type:Journal Article
- Keywords:
Hodgkin lymphoma;
Antigens, CD15;
Immunohistochemistry;
Sialic acids;
Fluoroimmunoassay
- From:
Chinese Journal of Pathology
2025;54(12):1282-1287
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of sialylated CD15 (CD15s) in the tumor cells of classical Hodgkin lymphoma using a modified immunohistochemical approach.Methods:From 2009 to 2024, 53 cases of classical Hodgkin lymphoma were collected in the Department of Pathology, Peking University Cancer Hospital, in which 21 cases that were CD15-negative or showed only focal weak positivity were selected. Immunohistochemical staining for CD15 was performed on a Leica automated stainer using three different antibody clones (MMA, Carb3, and IHC527). Tissue sections were digested with sialidase at varying concentrations and incubation times, followed by immunohistochemical staining with the MMA clone. Multiplex immunofluorescence was applied for co-staining of CD15 (MMA) and CD30 (JCM182), and analysis was conducted using APTIME and HALO software.Results:There were 30 male patients and 23 female patients, with an age range of 14 to 73 years and a median age of 32(26,46) years. None of the three CD15 antibody clones significantly improved the CD15 positive rate in the 14 completely negative and 7 weakly positive cases, with no notable differences observed among the clones( P>0.05). After sialidase digestion, tissue morphology remained well-preserved. Optimal CD15 staining was achieved with a 1∶1 diluted sialidase incubated at 37 ℃ for one hour. This treatment significantly enhanced the detection rate of CD15 antigen in Hodgkin Reed-Sternberg cells ( P<0.001). Conclusion:Sialidase digestion effectively unveils sialylated CD15 expression in classical Hodgkin lymphoma, markedly improving its detection in HRS cells.
