Consistency of claudin 18.2 expression with antibodies of different clones in gastric adenocarcinoma
10.3760/cma.j.cn112151-20250508-00331
- VernacularTitle:Claudin18.2抗体不同克隆号在胃腺癌组织中表达的一致性分析
- Author:
Chaoshan WANG
1
;
Dan ZHANG
;
Xin ZHANG
;
Fangcen LIU
;
Qianyun SHI
;
Hongyan WU
;
Hongping XIA
;
Qi SUN
;
Lin LI
Author Information
1. 南京大学医学院附属鼓楼医院病理科，南京210008
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Adenocarcinoma;
Tissue array analysis;
Antibodies, monoclonal;
Claudins;
Consistency analysis
- From:
Chinese Journal of Pathology
2025;54(11):1144-1149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the consistency of claudin 18.2 immunohistochemistry (IHC) using 4 different clone antibodies in gastric adenocarcinoma.Methods:A total of 226 gastric adenocarcinomas diagnosed and treated at the Drum Tower Hospital Affiliated to Nanjing University Medical College between January 2022 to March 2023 were included in this study. The cohort consisted of 165 males and 61 females, with a mean age of (61.3±12.1) years. Tumor tissues from radical resection specimens were collected for tissue microarrays. IHC detection of claudin 18.2 was performed using the EnVision method, utilizing 4 clones of antibody: OTIR157B5, 43-14A, EPR19202 and D313D22. The results were interpreted based on both the intensity of staining on tumor cell membranes and the percentage of positive tumor cells relative to the total tumor cells.Results:The positive cutoff value was set as moderately to strongly linear membrane staining in ≥75% of all viable invasive tumor cells, and clone OTIR157B5 demonstrated the highest positive expression rate at 52.2% (118/226). Additionally, the clones OTIR157B5, 43-14A, and EPR19202 were consistently and strongly positive, with all agreement rates of Cohen κ exceeding 0.8. In gastric adenocarcinoma and its three Lauren subtypes, OTIR157B5 exhibited clear membranous localization.Conclusions:Clone OTIR157B5 of claudin 18.2 antibody shows the highest rate of moderately to strongly linear membrane-positive staining, accounting for ≥75% of all viable invasive tumor cells, and clones 43-14A and EPR19202 show strong consistency and high sensitivity.
