Comprehensive Analysis of Flavonoid Content in Chrysanthemum Indicum:Germplasm Variation,Tissue Distribution,and Developmental Stage Changes
10.11842/wst.20240604005
- VernacularTitle:野菊黄酮类化合物含量综合分析:种质差异性、组织分布和发育阶段变化
- Author:
Jiahao LIAO
1
;
Haiqiong ZHANG
1
;
Zhiwei QIN
1
;
Xu WANG
1
;
Yifei LIU
1
;
Jingjing ZHANG
1
Author Information
1. 湖北中医药大学药学院 武汉 430065
- Publication Type:Journal Article
- Keywords:
Chrysanthemum indicum;
Germplasm resources;
Flavonoids;
Rhamnosyltransferase
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(6):1546-1554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide a scientific basis for the quality control of Chrysanthemi Indici Flos medicinal materials and the sustainable development of this resource,the germplasm differences,tissue distribution,and developmental stage changes of flavonoid content in Chrysanthemum indicum was explored.Methods High-performance liquid chromatography(HPLC)was used to quantitatively analyze the five main flavonoid compounds,including linarin,tilianin,cynaroside,apigenin,and acacetin.Real-time fluorescence quantitative PCR(qPCR)technology was used to evaluate the relative expression levels of the rhamnosyltransferase(RhaT)gene at different developmental stages.Results The study found significant differences in flavonoid accumulation among different Chrysanthemum indicum germplasms,with the highest content of linarin observed in the Hubei diploid.Flavonoid content was significantly higher in leaves,flower buds,and flowers compared to stems and roots.Linarin and tilianin peaked at the flower bud stage and then gradually decreased,while the contents of apigenin and acacetin increased with flower development.The qPCR analysis further showed that the expression level of the RhaT gene was highly consistent with the accumulation pattern of linarin.Conclusion The results reveal that flavonoid content in Chrysanthemum indicum is significantly affected by germplasm,tissue type,and developmental stage,providing important data support for the quality evaluation of Chrysanthemum indicum medicinal materials and the rational development of this resource.
