Resveratrol activates Wnt/β-catenin signaling pathway to inhibit apopto-sis and alleviate intestinal ischemia-reperfusion injury in mice
10.3969/j.issn.1000-4718.2025.06.019
- VernacularTitle:白藜芦醇通过激活Wnt/β-catenin信号通路抑制细胞凋亡而减轻小鼠肠缺血再灌注损伤
- Author:
Jialu MU
1
;
Yufang LENG
;
Xiaojie MA
;
Lingguo KONG
Author Information
1. 兰州大学第一临床医学院,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
resveratrol;
intestinal ischemia-reperfusion injury;
Wnt/β-catenin signaling pathway;
apoptosis
- From:
Chinese Journal of Pathophysiology
2025;41(6):1212-1217
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the mechanism by which resveratrol(RSV)alleviates intestinal ischemia-re-perfusion(I/R)injury through modulation of the Wnt/β-catenin signaling pathway.METHODS:Twenty-four male C57BL/6J mice were randomly allocated into four experimental groups:the sham group,I/R group,I/R+RSV(treatment)group,and I/R+RSV+XAV939(inhibitor)group,with 6 mice per group.In the I/R,treatment,and inhibitor groups,the superior mesenteric artery was clamped for 45 minutes and then reperfused for 2 hours to establish the intestinal I/R injury model.The sham group underwent vascular dissection without occlusion.The treatment and inhibitor groups received dai-ly intraperitoneal injections of 15 mg/kg resveratrol for 5 d preoperatively,and the other two groups were intraperitoneally injected with an equal volume of 0.1%dimethyl sulfoxide.The inhibitor group additionally received 10 mg/kg XAV939 30 minutes pre-ischemia.HE staining was used to observe the pathological changes in the intestinal tissues,and the Chiu score standard was used to evaluate the pathological damage.Apoptosis was evaluated through TUNEL staining and the apoptotic index(AI).Diamine oxidase(DAO)level was measured by ELISA.The expression levels of B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein,cleaved caspase-3,Wnt3a and β-catenin proteins were detected by Western blot.RESULTS:Compared with sham group,the mice in I/R group showed significantly increased Chiu score,elevated DAO level and AI(P<0.05),up-regulated cleaved caspase-3 and Bax protein levels,and down-regulated Bcl-2,Wnt3a and β-catenin protein levels(P<0.05).Treatment with RSV reversed these alterations(P<0.05).XAV939 co-administration abolished the protective effects of RSV(P<0.05).CONCLUSION:Resveratrol ameliorates intestinal I/R injury by acti-vating the Wnt/β-catenin signaling pathway and suppressing apoptosis.
