The effect of soluble receptor for advanced glycation end products on protein changes and function in cardiac ischemia-reperfusion based on proteomics
10.3969/j.issn.1006-7795.2025.03.015
- VernacularTitle:基于蛋白质组学揭示可溶性糖基化终末产物受体对缺血/再灌注心肌组织蛋白变化及功能的影响
- Author:
Xue JIANG
1
;
Panpan YU
;
Xiangjun ZENG
;
Caixia GUO
Author Information
1. 首都医科大学附属北京同仁医院心血管中心,北京 100730
- Publication Type:Journal Article
- Keywords:
sRAGE;
myocardial ischemia-reperfusion injury;
proteomics;
protein change;
function
- From:
Journal of Capital Medical University
2025;46(3):503-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of soluble receptor for advanced glycation end products(sRAGE)on the changes and functions of myocardial tissue proteins in mice during myocardial ischemia/reperfusion(I/R).Methods Establish the myocardial I/R model using cardiac-specific sRAGE transgenic mice,and apply proteomic methods to detect the types and levels of protein expression in myocardial tissue.Results Compared with the I/R+sRAGE KIfl/fl group,the I/R+sRAGE CKI group had 59 upregulated proteins and 42 downregulated proteins in myocardial tissue.The volcano plot showed that the significantly upregulated proteins were lghg1,lgh2b,Mcm7,and Nifk,and the significantly downregulated proteins were Abca7,Colla2,Ablim1,Crebrf,and Kcp,respectively.The subcellular localization results showed that the proteins with significant changes were mainly distributed in the nucleus,cytoplasm,extracellular space,plasma membrane,endoplasmic reticulum,cytoskeleton,and others.The results of functional enrichment showed that the significantly changed proteins were mainly involved in regulating signal transduction,cell motility,metabolism,infectious diseases,tumors and the immune system.Conclusion sRAGE can inhibit myocardial I/R injury by increasing or decreasing target proteins involved in regulating intracellular and extracellular signal transduction processes following myocardial I/R.
