A study on high glucose-activated CCAAT enhancer binding protein β up-regulated thyroid hormone nuclear receptor α1 expression and driving the pathogenesis of diabetic cardiomyopathy
10.3969/j.issn.1006-6187.2025.11.010
- VernacularTitle:高糖激活CCAAT增强子结合蛋白β促进甲状腺激素核受体α1表达驱动糖尿病心肌病发生的研究
- Author:
Xiaoni MA
1
;
Jiaojiao YANG
;
Liqiong YAO
;
Songbo FU
;
Chengxu MA
Author Information
1. 730000 兰州大学第一医院检验科
- Publication Type:Journal Article
- Keywords:
Hyperglycemia;
Diabetic cardiomyopathy;
CCAAT enhancer binding protein β;
Thyroid hormone nuclear receptor α1
- From:
Chinese Journal of Diabetes
2025;33(11):850-855
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hyperglycemia activated CCAAT enhancer binding protein β(C/EBPβ)promoting the expression of thyroid hormone nuclear receptor α1(THα1)on diabetic cardiomyopathy(DCM).Methods Twenty healthy male Wistar rats were randomly divided into diabetes mellitus(DM,n=10)group and DCM(n=10)group.Cardiomyocyte were divided into four groups:DM-cell,glucose intervention(Glu),knockdown C/EBPβ group(cardiomyocyte were transfected with plasmids targeting knockdown C/EBPβ)and si-NC group(cardiomyocyte were transfected with nagetive control sequence of plasmids).HE and Masson staining was performed to detect myocardial injury and collagen deposition.Echocardiography was used to evaluate left ventricular function.The mRNA and protein expression of C/EBP β and THα1 was detected using RT-qPCR,Western blot and immunofluorescence staining in myocardial cells.Apoptosis was analyzed using flow cytometric.Contraction frequency was counted by microscopy.Results Compared to DM group,count of fibroblasts,collagen volume fraction,end-systolic dimension of left ventricular,and the mRNA and protein expression of C/EBPβ and THα1 as well as fluorescence intensity increased(P<0.05);left ventricular ejection fraction and left ventricular fractional shortening(LVFS)decreased in DCM group(P<0.05).The mRNA and protein expression of C/EBPβ and THα1 were higher in the Glu group than in DM-cell group(P<0.05).The percentage of apoptosis,the mRNA and protein expression of THα1 of myocardial cells were lower in the si-C/EBPβ group than in the si-NC group(P<0.05),and LVFS was higher in the si-NC group(P<0.05).Conclusions High glucose regulates C/EBPβ expression,which in turn drives THα1 expression in myocardial tissue,leading to adverse effects on myocardial tissue.Conversely,silencing C/EBPβ downregulates THα1 expression,and exerts a protective effect on cardiomyocytes.
