Molecular biology techniques in the diagnosis of Mycobacterium abscessus disease
10.3760/cma.j.issn.1674-2397.2025.01.006
- VernacularTitle:分子生物学技术对脓肿分枝杆菌病的诊断价值
- Author:
Pinru CHEN
1
;
Hua CHEN
1
;
Jialou ZHU
1
Author Information
1. 广州市胸科医院非结核分枝杆菌病诊疗中心，广州 510095
- Publication Type:Journal Article
- Keywords:
Mycobacterium;
Mycobacterium abscess complex;
Molecular biology techniques;
Genotype
- From:
Chinese Journal of Clinical Infectious Diseases
2025;18(1):37-46,76
- CountryChina
- Language:Chinese
-
Abstract:
In recent year，the iatrogenic infections and outbreaks caused by Mycobacterium abscessus complex（MABC）have been increasingly reported. Studies indicate that MABC accounts for 65%-80% of rapidly growing non-tuberculosis Mycobacterium（NTM）-related pulmonary diseases. As a clinically significant NTM pathogen，MABC exhibits rapid proliferation and intrinsic resistance to multiple antimicrobial agents. Notably，the 16S rDNA sequences among MABC subspecies share 100% homology，posing challenges for subspecies-level differentiation. Rapid and precise identification of MABC at the species or subspecies level is therefore critical for effective clinical management and outbreak control. Conventional biochemical methods for NTM identification are time-consuming and prone to inaccuracies，whereas molecular biology techniques offer superior speed and specificity，and have been widely used clinically. This review highlights the clinical implications of molecular biological techniques in MABC identification and its pivotal role in guiding therapeutic strategies.
