Metagenomic next-generation sequencing in diagnosis of non-tuberculous mycobacterial bone and joint infections
10.3760/cma.j.issn.1674-2397.2025.01.004
- VernacularTitle:宏基因组二代测序在非结核分枝杆菌骨关节感染中的诊断价值
- Author:
Guangxuan YAN
1
;
Xueyu WANG
;
Wenjuan NIE
Author Information
1. 首都医科大学附属北京胸科医院骨科，北京市结核病胸部肿瘤研究所，北京 101149
- Publication Type:Journal Article
- Keywords:
Bone and joint infection;
Non-tuberculous mycobacteria;
Metagenomic next-generation sequencing;
Diagnosis
- From:
Chinese Journal of Clinical Infectious Diseases
2025;18(1):25-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the diagnostic value of metagenomic next-generation sequencing（mNGS）in bone and joint infections caused by non-tuberculous mycobacteria（NTM）.Methods:Clinical data of 175 patients with suspected NTM bone and joint infections admitted in Beijing Chest Hospital，Capital Medical University from January 2019 to January 2023 were retrospectively analyzed. Mycobacterium growth indicator tube（MGIT）method was used for mycobacterial culture on the bone tissue or abscess samples and mNGS test was performed on bone tissue samples in all patients. Taking clinical diagnosis as the gold standard，the sensitivity，specificity，positive predictive value，negative predictive value，positive likelihood ratio，and negative likelihood ratio of the two methods were compared.Results:Twenty-six patients（14.9%）were clinically diagnosed as NTM bone and joint infections. The mNGS showed higher sensitivity（100.0% vs. 57.7%，），specificity（99.3% vs. 86.6%），positive predictive value（96.3% vs. 42.9%），and negative predictive value（100.0% vs. 92.1%），compared to MGIT culture（all P<0.001）. The positive likelihood ratio（149.00 vs. 4.31）and negative likelihood ratio（0 vs. 0.49）of mNGS were also superior to those of MGIT. Conclusion:Compared to MGIT culture，mNGS has high diagnostic value in NTM bone and joint infections and can serve as an efficient and reliable method for clinical diagnosis.
