Role of exosomes on regulatory T cells after radiation irradiation for triple-negative breast cancer cells
10.3760/cma.j.cn113030-20240611-00227
- VernacularTitle:放射线照射三阴性乳腺癌细胞后外泌体对Treg细胞的作用
- Author:
Jinli REN
1
;
Chi PAN
;
Qingtao NI
Author Information
1. 南京医科大学附属泰州人民医院针灸理疗科，南京医科大学泰州临床医学院，泰州 225300
- Publication Type:Journal Article
- Keywords:
Exosomes;
Triple negative breast neoplasms;
Radiotherapy;
T-lymphocytes, regulatory
- From:
Chinese Journal of Radiation Oncology
2025;34(3):289-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of exosomes released from triple-negative breast cancer cells (TNBC) on immune cells after radiotherapy.Methods:When TNBC (3 types: MDA-MB-231, MDA-MB-453, MDA-MB-468) reached 70% confluence, cells were irradiated with a dose of 8 Gy in one group and no intervention was given in the control group. The supernatants were collected at 48 h after irradiation. Subsequently, these supernatants were co-cultured with lymphocytes in peripheral blood mononuclear cells, and the uptake of exosomes by T cells was confirmed by fluorescence microscopy. Meanwhile, the expression of regulatory T cells (Treg) in the cells was detected using flow cytometry. Differences in Treg cell differentiation between two groups were compared by t-test (expressed as Treg cell positivity rate). Results:Transmission electron microscopy scanning and nanoparticle analysis showed that the extracellular vesicles extracted in the experiment were exosomes. Lymphocytes phagocytosed the exosomes and the exosomes were mainly concentrated in the cytoplasm after phagocytosis. Following the co-culture of 3 kinds of lymphocytes with exosomes from TNBC, there was an increase in Treg cell differentiation compared to control group (1.07%, 0.60%, 0.63% vs. 0.30%, P<0.01). In addition, exosomes released from TNBC further increased the differentiation of Treg cells after radiotherapy (MDA-MB-231 cells: 1.07% vs. 1.81%, P<0.01; MDA-MB-453 cells: 0.60% vs. 0.93%, P<0.05). Conclusions:In summary, exosomes released from TNBC can promote the differentiation of Treg cells. In addition, radiotherapy-induced exosomes released by TNBC further exacerbate the differentiation of Treg cell.
