Effects of DP1 receptor agonist on expression of cytokines and injury-related fac-tors in bovine bone marrow-derived macrophages stimulated by E.coli
10.16303/j.cnki.1005-4545.2025.10.10
- VernacularTitle:DP1受体激动剂对大肠杆菌诱导奶牛骨髓源巨噬细胞中细胞因子和损伤相关因子表达的影响
- Author:
Jingze WU
1
;
Xiaolin YANG
1
;
Pengfei GONG
1
;
Lili GUO
1
;
Jiahui YU
1
;
Wei MAO
1
;
Shuangyi ZHANG
1
;
Bo LIU
1
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;农业农村部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特 010018
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
macrophages;
prostaglandin D2;
DP1 receptors
- From:
Chinese Journal of Veterinary Science
2025;45(10):2163-2169
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the effect of PGD2/DP1 receptor pathway on the expression of cyto-kines and injury-related factors in Escherichia coli(E.coli)induced bovine bone marrow derived macrophages,an in vitro model of E.coli induced bovine bone marrow derived macrophages was established.The effects of DP1 receptor agonist on phagocytosis and killing ability,mRNA expres-sion,secretion of pro-inflammatory cytokines(TNF-α,IL-1β)and activation of signaling pathway(MAPK,NF-κB)in cow bone marrow derived macrophages induced by E.coli were examined.The results showed that compared with the E.coli infection group,the phagocytosis and killing ability of BW-245C+E.coli group and 15 d-PGJ2+E.coli group were enhanced(P＜0.01).Compared with the blank control group,mRNA expression was at a higher level(P＜0.001),and the secre-tion of pro-inflammatory cytokines(TNF-α,IL-1β)was significantly increased after adding E.coli solution.The mRNA expression of BW-245C+E.coli group and 15 d-PGJ2+E.coli group were significantly decreased(P＜0.001),and the secretion of pro-inflammatory cytokines(TNF-α,IL-1β)was significantly decreased(P＜0.001).and signaling pathway(MAPK,NF-κB)were sig-nificantly down-regulated(P＜0.001).This study showed that DP1receptor agonist plays an inhib-itory role in the inflammatory response of cow bone marrow-derived macrophages induced by E.coli.This finding provides a potential target for future treatment of cow endometritis,laying the foundation for the development of novel anti-inflammatory treatment strategies.
