Effects of hepatitis B virus genotypes B and C on hepatic stellate cells under different alanine aminotransferase statuses
10.12007/j.issn.0258-4646.2025.06.004
- VernacularTitle:不同丙氨酸氨基转移酶状态下B/C基因型乙型肝炎病毒对肝星状细胞的影响
- Author:
Peng GAO
1
;
Longdong ZHU
;
Junfeng LI
;
Liqiong YAO
Author Information
1. 兰州大学第一医院医学检验中心,兰州 730000
- Publication Type:Journal Article
- Keywords:
hepatitis B;
hepatic stellate cell;
alanine aminotransferase;
cell cycle;
cell proliferation;
cell secretion
- From:
Journal of China Medical University
2025;54(6):500-504,509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hepatitis B virus genotypes B and C on hepatic stellate cells(HSC)proliferation,cell cycle,and secretion under different alanine aminotransferase(ALT)statuses.Methods A total of 270 patients diagnosed with chronic hepatitis B in the First Hospital of Lanzhou University between January 2023 to December 2023 were selected by stratified random sam-pling.In the group with normal ALT,there were 42 patients with genotype B and 48 patients with genotype C.In the group with ALT ≥ the upper normal limit(UNL)and＜2 times the UNL(2 UNL),there were 48 patients with genotype B and 45 patients with genotype C.In the group with ALT≥2 UNL,there were 45 patients with genotype B and 42 patients with genotype C.Sera from these patients was used as the interfering sera.After HSC were treated with the interfering serum,the proliferation of HSC was detected by the MTT method,the cell cycle was detected by flow cytometry,the mRNA expressions of TGF-β1,Smad3,Smad7,α-SMA,Collagen Ⅰ,and Collagen Ⅲ were detected by real-time PCR,and the expressions of the corresponding proteins were detected by Western blotting.Results When the ALT level was normal,hepatitis B virus genotypes B and C had no obvious effect on HSCs.When the ALT level was abnormal,particularly in the group with ALT ≥ 2 UNL,the virus with genotype C was more likely to promote the proliferation of HSC,reduce the number of cells in the G0/G1 phase,increase the number of cells in the S phase and G2/M phase,enhance the mRNA and protein expressions of TGF-β1,Smad3,Collagen Ⅰ,Collagen Ⅲ,and α-SMA,and decrease the mRNA and protein expressions of Smad7,with significant differences(P＜0.05).Conclusion The effects of hepatitis B virus genotypes B and C on HSC differ varying ALT statuses.During abnormal ALT elevation,genotype C hepatitis B virus demonstrates more pronounced effects on HSC proliferation,cell cycle progression,and secretory function compared to genotype B.
