Screening of ferroptosis biomarkers associated with acute myocardial infarction based on weighted gene co-expression network analysis
10.3969/j.issn.1008-0074.2025.03.19
- VernacularTitle:基于加权基因共表达网络分析筛选与急性心肌梗死相关的铁死亡生物标记物
- Author:
Hong-zhen WU
1
;
Hong-yan WANG
1
Author Information
1. 福建医科大学孟超肝胆医院心内科,福建 福州 350001
- Publication Type:Journal Article
- Keywords:
Myocardial infarction;
Genes;
Ferroptosis
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2025;34(3):385-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Based on the dataset in the Gene Expression Omnibus database(GEO),we screened out the co-expression modules most relevant to acute myocardial infarction(AMI)and identified ferroptosis-related bio-markers.Methods:We constructed a weighted gene co-expression network for the hypervariable genes in the GSE123342 dataset,screened out the gene modules most related to AMI and identified ferroptosis-related biomark-ers from the key modules,and then observed their expression levels in tissues as well as further analyzed their corre-lation with the immune microenvironment.Results:One key module most relevant to AMI patients was obtained.By intersecting with differential genes,a total of 532 differential genes that were up-regulated in AMI were ob-tained.Functional enrichment analysis showed that they were mainly enriched in biological processes such as granu-locyte migration,reactive oxygen species generation,leukocyte chemotaxis and activation,and defense response regulation.The pathways were mainly enriched in Toll-like signaling pathway,nuclear factor kappa B(NF-κB)signaling pathway,tumor necrosis factor(TNF)signaling pathway,hypoxia-inducible factor-1(HIF-1)signa-ling pathway and cytokine-cytokine receptor interaction pathways,etc.Finally,14 genes were found that were promising targets for AMI treatment:zinc finger antisense 1(ZFAS1),WD repeat domain,phosphoinositide inter-acting 1(WIPI1),Toll-like receptor 4(TLR4),quiescin sulfhydryl oxidase 1(QSOX1),phosphatase and tensin homolog(PTEN),phosphogluconate dehydrogenase(PGD),mitochondrial fusion protein 2(MFN2),double mi-nute 2 homolog(MDM2),mitogen-activated protein kinase 14(MAPK14),mitogen-activated protein kinase 1(MAPK1),gamma-aminobutyric acid receptor-associated protein-like 1(GABARAPL1),arachidonate 5-li-poxygenase(ALOX5),long-chain acyl-CoA synthetase 4(ACSL4),and long-chain acyl-CoA synthetase 1(ACSL1).Correlation analysis revealed that they all had obvious correlations with various immune cells.Conclusion:The 14 ferroptosis genes associated with AMI are expected to become potential immunotherapy targets.
