Expression and significance of RANK/RANKL/OPG signalling pathway in gerbil external ear canal cholesteatoma
10.3969/j.issn.1006-7299.2025.06.013
- VernacularTitle:RANK/RANKL/OPG信号通路在沙鼠外耳道胆脂瘤中的表达及意义
- Author:
Guanwen HE
1
;
Xiaoqing LIN
;
Xiuqin GUO
;
Wenxian LU
Author Information
1. 福建医科大学宁德临床医学院(宁德 352100);宁德师范学院附属宁德市医院耳鼻咽喉科福建省宁德市耳鼻咽喉科研究所(宁德 352100);宁德市医院医疗集团屏南县总医院耳鼻咽喉科(宁德 352300)
- Publication Type:Journal Article
- Keywords:
External auditory canal cholesteatoma(EACC);
Bone destruction;
Receptor activator of NF-κB(RANK);
Receptor activator of NF-κB ligand(RANKL);
Osteoprotegerin(OPG)
- From:
Journal of Audiology and Speech Pathology
2025;33(6):575-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of RANK/RANKL/OPG signaling path-way in gerbil external auditory canal cholesteatoma(EACC).Methods Twenty-five mongolian long-clawed gerbils were divided into an experimental group(n=15)and a control group(n=10),and an EACC model was constructed by external auditory canal ligation method in the experimental group.Three months later,the external auditory ca-nal tissues of the control group and the experimental group(10 ears)were collected,and RT-qPCR and Western blot were performed to detect the expression levels of receptor activator of NF-κB(RANK),receptor activator of NF-κB ligand(RANKL),osteoprotegerin(OPG)mRNA and protein in the signaling pathway.Results Two ger-bils in the experimental group died during the modeling process,and cholesteatoma of the external auditory canal was formed in 10 ears 3 months after the operation,with a modeling success rate of 76.9％(10/13).Compared with the normal control group,there was no statistically significant difference in the expression of RANK mRNA in gerbil external auditory canal tissues(P=0.08).The expression of RANK protein was upregulated,the expression of RANKL mRNA and protein was upregulated,while the expression of OPG mRNA and protein was downregulat-ed,the ratio of RANKL/OPG was upregulated,with a statistically significant difference between the two groups(P＜0.01).Conclusion The RANK/RANKL/OPG signaling system may play an important role in the formation of gerbil EACC and bone destruction.
