Mechanism of tetrahydrocurcumin in improving kidney injury in mice with type 2 diabetes mellitus based on transcriptomics
10.12007/j.issn.0258-4646.2025.06.003
- VernacularTitle:基于转录组学探讨四氢姜黄素改善2型糖尿病小鼠肾损伤的作用机制
- Author:
Junyu MA
1
;
Chunmei ZHANG
;
Yang JIANG
;
Mengyao LI
;
Xiaoyan BI
;
Fuli YA
Author Information
1. 大理大学公共卫生学院营养与食品卫生学教研室,云南大理 671000
- Publication Type:Journal Article
- Keywords:
tetrahydrocurcumin;
renal injury;
transcriptomics;
type 2 diabetes mellitus
- From:
Journal of China Medical University
2025;54(6):493-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how dietary supplementation with tetrahydrocurcumin(THC)improves kidney injury in type 2 dia-betic mellitus(T2DM)and its mechanism of action using transcriptome sequencing(RNA-seq).Methods C57BL/6J mice were randomly assigned to the control,T2DM,and T2DM+THC groups.After a high-fat meal and streptozotocin injection,the body weights and fasting blood glucose levels of each mouse with T2DM were measured.Hematoxylin and eosin staining,Oil red O staining,and RNA-seq were performed to examine kidney pathology,lipid deposition,and differentially expressed genes,respectively,in the mice.Results Mice in T2DM group exhibited significantly higher fasting blood glucose levels(P＜0.001),renal tubule degeneration,glomeruli enlargement,disordered epithelial cells,and increased kidney lipid deposition after 12 weeks compared with those of the control group.THC adminis-tration alleviated all these conditions(P＜0.001).RNA-seq analysis revealed significant gene expression variations among the control,T2DM,and T2DM+THC groups.THC may protect against T2DM-induced kidney injury and lipid deposition by regulating the cell cycle(apoptosis),P53 signaling pathway,and PPARγ signaling path way,as indicated by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.In mice with T2DM,THC intervention may upregulate the expression of Cd36,Lpl,PPARγ,and Plin4 genes in renal tissues,while downregulating Ccnb1,Ccnb2,Cdk1,Bub1,and Cdc25c gene expressions.The proteins encoded by the four upregulated genes interact,as do those encoded by the five downregulated genes.Conclusion THC administration improves fasting blood glucose levels,reduces renal damage,and decreases fat deposition in mice with T2DM.The processes may involve decreasing apoptosis,blocking the P53 signaling pathway,and activating the PPARγ signaling pathway.
