Comparative study of idiopathic pulmonary fibrosis in mice induced by bleomycin of different concentrations
10.3969/j.issn.1005-4847.2025.05.006
- VernacularTitle:不同浓度博来霉素诱导特发性肺纤维化小鼠模型的比较研究
- Author:
Xinxin ZHANG
1
;
Yizi XIE
;
Huiting HUANG
;
Yong JIANG
;
Hang LI
;
Gang LIAO
;
Wujin WEN
;
Shaofeng ZHAN
;
Xiufang HUANG
Author Information
1. 广州中医药大学第一附属医院,广州 510000;广州中医药大学第一临床医学院,广州 510000;广州中医药大学岭南医学研究中心,广州 510000;广东省中医临床研究院,广州 510000
- Publication Type:Journal Article
- Keywords:
idiopathic pulmonary fibrosis;
bleomycin;
animal model;
intratracheal infusion
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(5):673-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the establishment and evaluation of an idiopathic pulmonary fibrosis(IPF)mouse model induced by the intratracheal infusion of bleomycin(BLM)of different concentrations.Methods Male C57BL/6J mice were randomly divided into a control group,Model-L group(1.5 mg/kg,BLM),Model-M group(2.5 mg/kg,BLM),and Model-H group(3.5 mg/kg,BLM).An IPF mouse model was constructed by one-time intratracheal infusion of BLM.The general status,body mass,survival rate,and lung coefficient of mice in different groups were compared.Pathological changes in lung tissue,the hydroxyproline content,fibrosis markers and inflammatory factor levels were observed.Results Compared with the control group,the survival rate decreased and body weight showed a downward trend in the low-,medium-,and high-dose model groups,with significant increases in lung coefficients.Inflammatory infiltration(P＜0.01)and collagen deposition(P＜0.0001)were observed in the lung tissues of all model groups.Hydroxyproline levels in lung tissue and serum were significantly elevated(P＜0.05).The mRNA levels of fibrosis markers α-Sma,Fn1,and Col1a1 were upregulated(P＜0.001),with significant increases in corresponding protein expression(P＜0.05).The mRNA expression of the inflammatory factor Tgfb1 also increased(P＜0.0001).Conclusion 1.5,2.5 and 3.5 mg/kg BLM can induce an IPF model in C57BL/6J mice.Based on the results observed for survival rate,body mass,lung coefficient changes,lung tissue gross and pathological changes,and fibrosis-related biomarkers,2.5 mg/kg BLM is the optimal concentration for inducing an IPF mouse model.
