Experimental study on sleep deprivation inhibiting clock gene CRY1 expression in vascular tissue and promoting vascular senescence
10.20039/j.cnki.1007-3949.2025.05.004
- VernacularTitle:睡眠剥夺抑制大鼠血管生物钟因子CRY1表达促进血管衰老的实验研究
- Author:
Jialong NIU
1
;
Furong WANG
;
Kexin WANG
;
Wenjie WANG
;
Yixuan LIU
;
Xiaoyi MA
;
Zhongke WANG
;
Hailong GE
Author Information
1. 首都医科大学附属北京安贞医院心内科北京市心肺血管疾病研究所,北京市 100000
- Publication Type:Journal Article
- Keywords:
sleep deprivation;
vascular senescence;
cryptochrome 1;
clock gene;
rat
- From:
Chinese Journal of Arteriosclerosis
2025;33(5):395-401
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the relationship between sleep deprivation and vascular aging,as well as the un-derlying mechanisms.Methods Male Sprague-Dawley rats were divided into control group,senescence group,sleep deprivation group,and sleep deprivation+senescence group,with 6 rats in each group.The modified level table method deprived rats of sleep duration.Senescence-associated β-galactosidase(SA-β-Gal)staining was used to detect the senes-cence status of rat vascular tissue.The mRNA and protein expression of tumor suppressor protein p53,silent information regulator 1(SIRT1)and clock gene cryptochrome 1(CRY1)was detected by real-time fluorescence quantification PCR(RT-qPCR),Western blot and immunohistochemistry.Results Compared with the control group,the intensity of SA-β-Gal staining was increased in the vascular tissues of the senescence group rats,the expression level of p53 was elevat-ed,the expression level of SIRT1 was decreased.Similar changes were observed in the sleep deprivation group and the sleep deprivation+senescence group,including intensified SA-β-Gal staining,elevated p53 levels,and reduced SIRT1 lev-els in vascular tissues.Additionally,compared with the control group,the sleep deprivation group showed reduced CRY1 levels in vascular tissues,while only CRY1 mRNA levels were reduced in the sleep deprivation+senescence group.Fur-thermore,compared with the senescence group,the sleep deprivation+senescence group exhibited intensified SA-β-Gal staining,increased p53 level,decreased SIRT1 level,and reduced CRY1 mRNA level in vascular tissues.Conclusion Sleep deprivation may promote the expression of vascular aging-related factors,potentially through the inhibition of CRY1 expression in vascular tissues.
