Protective effect and mechanism of baicalin on vascular endothelial function in young rats with diabetes mellitus by regulating oxidative stress response
10.3969/j.issn.1006-6187.2025.06.011
- VernacularTitle:黄芩苷调控氧化应激反应对糖尿病幼鼠血管内皮功能保护作用及机制的研究
- Author:
Yueyun LI
1
;
Xiaofang WANG
;
Yan FU
Author Information
1. 453000 新乡市中心医院儿科
- Publication Type:Journal Article
- Keywords:
Baicalin;
Diabetes mellitus;
Vascular endothelial function;
Oxidative stress
- From:
Chinese Journal of Diabetes
2025;33(6):462-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of baicalin(Bai)on vascular endothelial function in young rats with diabetes mellitus by regulating oxidative stress response.Methods 60 SD young rats were randomly divided into control(NC)group,model(Mod)group,Bai 50 mg/kg(Bai 50)group,Bai 100 mg/kg(Bai 100)group,Bai 200 mg/kg(Bai 200)group,and inhibitor(Bai 200+ML-385)group,with 10 rats in each group.Fasting blood glucose(FPG),nitric oxide(NO),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)were compared among all groups.Aortic pathology was evaluated by HE staining,vasodilation rate was evaluated by in vitro vascular ring assay,and reactive oxygen species(ROS)was evaluated by DHE staining.Western blot was used to detect protein expression of nuclear factor E2 related factor 2(Nrf2),heme oxygenase 1(HO-1),and endothelial nitric oxide synthase(eNOS)in aortic tissue.Results Compared with the NC group,the levels of FPG,MDA,and ROS in the Mod group were increased(P<0.05),the NO,SOD,GSH-Px,Ach-induced vasodilation rate,p-Nrf2/Nrf2,HO-1,and eNOS protein expression were decreased in the Mod group(P<0.05).Compared with the Mod group,the levels of FPG,MDA,and ROS in the Bai 50,Bai 100,and Bai 200 groups were decreased(P<0.05),the NO,SOD,GSH-Px,ACh-induced vasodilation rate,p-Nrf2/Nrf2,HO-1,while and eNOS protein expression were increased in the Bai 50,Bai 100,and Bai 200 groups(P<0.05).Bai 200+ML-385 group reversed the protective effect of Bai 200 on vascular endothelial function in young rats with DM.Conclusions Bai can protect vascular endothelial function in young rats with DM,and its mechanism may be related to the activation of Nrf2/HO-1 signaling pathway and antioxidant stress response.
