Anti-fatigue effect of chicory polysaccharide on mice exposed to simulated hypobaric hypoxia and mechanisms
10.3867/j.issn.1000-3002.2025.08337
- VernacularTitle:菊苣多糖对低压低氧暴露小鼠的抗疲劳作用及机制
- Author:
Yingxian SHI
1
;
Shuo LI
;
Yulong HUANG
;
Xin CHENG
;
Dingduo SHAN
;
Youzhi ZHANG
;
Henglin WANG
Author Information
1. 河北北方学院研究生学院,河北 张家口 075000;解放军总医院第六医学中心麻醉科,北京 100048;军事医学研究院,北京 100850;国家安全特需药品全国重点实验室,北京 100039
- Publication Type:Journal Article
- Keywords:
chicory polysaccharide;
high altitude;
hypobaric hypoxia;
fatigue;
energy metabolism;
signaling pathway
- From:
Chinese Journal of Pharmacology and Toxicology
2025;39(10):742-750
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the anti-fatigue effect of chicory polysaccharide(CP)on mice exposed to simulated hypobaric hypoxia.METHODS Male C57BL/6J mice were randomly divided into the control group,model group,model+CP 150,300 and 600 mg·kg-1 groups.The control and model groups were given normal saline,while the CP groups were given drugs of different doses.After a 14 d pre-administration period,all the mice except the control group were exposed to a simulated alti-tude of 7 000 m in a hypobaric and hypoxic animal experimental chamber.After 7 d,a treadmill fatigue test was conducted to assess exercise endurance.The body weight and organ indexes were evaluated.The pathological changes in organs and tissues were observed via HE staining.The levels of fatigue-related and oxidative stress-related indicators were measured.The expression levels of phosphorylated AMP-activated protein kinase(p-AMPK),peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1α),and cytochrome c oxidase Ⅳ(COXⅣ)were determined using Western blotting anal-ysis.RESULTS Compared with model group,exercise endurance was significantly enhanced,body weight and organ indexes improved,and pathological damage to the lung,liver and skeletal muscle mitigated in the model+CP 600 mg·kg-1 group.Compared with model group,the model+CP 600 mg·kg-1 group had the contents of serum lactate and blood urea nitrogen reduced,but the contents of glycogen and the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the liver and skeletal muscle were increased.The malondialdehyde content was lowered,but the expressions of p-AMPK,PGC-1α,and COXⅣ in skeletal muscle were significantly increased.CONCLUSION CP can alleviate altitude-induced fatigue by reducing the metabolite accumulation,increasing glycogen storage,and lowering oxidative stress levels.The underlying mechanism may involve the activation of the AMPK/PGC-1αsignaling pathway.
