An Exploratory Experiment on the Dynamic Structural Change of ATP Synthase
10.13865/j.cnki.cjbmb.2025.02.1467
- VernacularTitle:ATP合酶动态结构的开放探索性实验教学
- Author:
Yi-Xuan LIU
1
;
Yang LIU
;
Wen-Yuan ZHU
;
Xiao-Qian HU
;
Zeng-Yi CHANG
;
Yong-Mei QIN
;
Qing-Song WANG
Author Information
1. 北京大学生命科学学院,生物学国家级实验教学示范中心
- Publication Type:Journal Article
- Keywords:
ATP synthase;
dynamic structural change;
photocrosslinking;
high-throughput electropho-resis;
exploratory experiment
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(5):625-631
- CountryChina
- Language:Chinese
-
Abstract:
The lab module of exploratory experiment is newly designed in the practical course of bio-chemistry.Here we describe one of the experimental projects,and it originates from new scientific re-search results on the dynamic structure of ATP synthase.This exploratory experiment is organized in the form of real scientific research,which would fully mobilize the initiative and creativity of students in learning theoretical knowledge and experimental technology.Students work in groups and start with refer-ence reading.Through cooperation,they must develop certain experimental plan,handle samples with photocrosslinking technique and utilize the high-throughput electrophoresis method to analyze the dynamic structural change of ε subunit in ATP synthase under different physiological conditions.High quality re-sults from high-throughput electrophoresis can only be obtained through optimized operation and treat-ment,from which students would experience the process of technological innovation.The teaching process of this lab module embodies the student-centered teaching concept and is widely approved and supported by students.The project of ATP synthase closely combines the content of lab course with cut-ting-edge technology.Students can deeply experience the importance of experimental technology innova-tion in solving scientific problems.The practical ability of students would be comprehensively improved through this lab module.
