Lipidomics-Based Study on the Mechanism of Sanhan Huashi Formula in Treating Respiratory Syncytial Virus Pneumonia Model Mice
10.14148/j.issn.1672-0482.2025.1480
- VernacularTitle:基于脂质组学探究散寒化湿方治疗呼吸道合胞病毒肺炎模型小鼠的作用机制
- Author:
Yuqing ZHU
1
;
Keyu TAO
;
Yingcai XIONG
;
Jiru ZHANG
;
Zhaowei ZHANG
;
Xiaoqing ZHOU
;
Jiani TAN
;
Jianjian JI
Author Information
1. 南京中医药大学第一临床医学院,江苏 南京 210023;儿童健康与中医药省高校重点实验室,江苏 南京 210023;南京中医药大学医学院,江苏 南京 210023
- Publication Type:Journal Article
- Keywords:
Sanhan Huashi Formula;
respiratory syncytial virus;
pneumonia;
lipidomics;
ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry;
host immunity;
lipids
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(11):1480-1492
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the therapeutic effect of Sanhan Huashi Formula(SHF)on respiratory syncytial virus(RSV)-infected mouse models and explore its potential antiviral and anti-inflammatory mechanisms using lipidomics.METHODS Fifty-four BALB/c mice were randomly divided into six groups(n=9):blank group,model group,Ribavirin group(50 mg·kg-1·d-1),and SHF high(15.46 g·kg-1·d-1),medium(7.73 g·kg-1·d-1),and low-dose(3.87 g·kg-1·d-1)groups.A pneumonia model was established by in-tranasal RSV infection,followed by three consecutive days of oral gavage administration.Lung tissues were collected for histopathologi-cal evaluation using hematoxylin-eosin(HE)staining and inflammation scoring.Real-time quantitative polymerase chain reaction(RT-qPCR)was performed to measure mRNA levels of viral gene fusion protein(F),glycoprotein(G),and inflammatory factors tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)to assess lung viral load and inflammation,while immunofluorescence staining was performed to observe the expression of RSV-F protein in lung tissues.Serum lipidomics analysis was conducted using ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry(UPLC-Q Exactive Or-bitrap MS)to identify lipid metabolism changes and differential lipids.RESULTS Compared with the blank group,mice in the model group exhibited marked pulmonary inflammatory cell infiltration and tissue injury,with significantly elevated pulmonary histopa-thology scores and lung index.The lung viral load and the mRNA expression levels of the inflammatory factors TNF-α and IL-6 were significantly increased,and immunofluorescence likewise indicated high expression of RSV-F protein in lung tissue.Relative to the model group,treatment with SHF at all tested doses clearly ameliorated lung tissue injury,effectively suppressed viral gene expression and inflammatory cytokine levels,and reduced the fluorescence signal intensity of RSV-F protein in the lungs.Lipidomics analysis re-vealed that compared with the blank group,the model group exhibited marked disturbances in lipid metabolism-characterized by dys-regulation of triacylglycerol(TG),phosphatidylcholine(PC),lysophosphatidylcholine(LPC),sphingomyelin(SM),diacylglycerol(DG),lysophosphatidylethanolamine(LPE),and phosphatidylethanolamine(PE).High-dose SHF treatment reversed these RSV-induced lipid abnormalities.CONCLUSION SHF effectively alleviates RSV-induced pulmonary inflammation and pathological injury,re-duces pulmonary RSV viral load,and may exert these effects by modulating dysregulated lipid metabolism in peripheral blood.
