let-7 inhibits proliferation,migration,and invasion of oral squamous cell carcinoma cells by targeting and regulating Wnt/β-catenin pathway
10.12007/j.issn.0258-4646.2025.06.012
- VernacularTitle:let-7靶向调控Wnt/β-catenin通路抑制口腔鳞状细胞癌细胞的增殖、迁移和侵袭
- Author:
Xiaoying LIU
1
;
Yanxin WANG
;
Chunbo SU
;
Xin LIU
;
Yanhao DUAN
;
Jiahong ZHAO
;
Yongle QIU
Author Information
1. 石家庄市第三医院口腔科,石家庄 050000
- Publication Type:Journal Article
- Keywords:
oral squamous cell carcinoma;
let-7;
Wnt/β-catenin;
proliferation;
invasion
- From:
Journal of China Medical University
2025;54(6):547-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the role of let-7 in the pathogenesis of oral squamous cell carcinoma(OSCC)and its underlying molecular mechanism.Methods The expression of let-7 in human oral keratinocyte(HOK),OSCC tissues,and OSCC cells(CAL-27,SCC-25,and SCC-9)was detected by quantitative real-time PCR.Colony formation,scratch,Transwell,and flow cytometry assays were used to examine the effects of let-7 on the proliferation,migration,invasion,and apoptosis of SCC-25 cells.Western blotting was used to detect the expression of Wnt and β-catenin proteins.The correlation between let-7 expression and the clinicopathological cha-racteristics and prognosis of OSCC patients was analyzed.Results let-7 was downregulated in OSCC tissues and cells.let-7 over-expression effectively inhibited the proliferation,migration,and invasion of SCC-25 cells while promoting apoptosis by suppressing acti-vation of the Wnt/β-catenin pathway.let-7 expression was closely associated with tumor stage,lymph node metastasis,and patient survival status.Patients with low let-7 expression exhibited lower overall survival rate.Conclusion let-7 targets and regulates the Wnt/β-catenin pathway,thereby inhibiting proliferation,migration,and invasion of OSCC cells while promoting apoptosis.
