Effects of host circular RNA circCAMSAP2 on proliferation of porcine deltacoro-navirus
10.16303/j.cnki.1005-4545.2024.12.04
- VernacularTitle:宿主环形RNA circCAMSAP2对猪丁型冠状病毒增殖的影响
- Author:
Sujing DIAO
1
;
Liuyang DU
1
;
Tao WAN
1
;
Jiyong ZHOU
1
;
Jinyan GU
1
Author Information
1. 浙江大学 动物医学中心,浙江 杭州 310058;浙江大学农业农村部动物病毒学重点实验室,浙江 杭州 310058
- Publication Type:Journal Article
- Keywords:
circular RNA;
circCAMSAP2;
PDCoV;
virus proliferation
- From:
Chinese Journal of Veterinary Science
2024;44(12):2526-2533
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study is to investigate the impact of host circular RNA(circRNA)on the proliferation of porcine deltacoronavirus(PDCoV).Among the significantly downregulated cir-cRNAs identified during PDCoV infection from circRNA expression profiles in ST cells,circCAM-SAP2 was selected for further analysis.The gene origin and junction sequence of circCAMSAP2 were validated using the online Ensembl database and Sanger sequencing.The molecular character-istics of circCAMSAP2 were determined through nucleoplasmic separation assays and RNase R tests.Additionally,the differential expression of circCAMSAP2 during PDCoV infection was con-firmed using RT-qPCR.Overexpression and knockdown experiments were conducted to investigate the regulatory role of circCAMSAP2 in PDCoV replication.The results indicated that circCAM-SAP2 is a circRNA derived from exon 2 of the CAMSAP2 gene through reverse splicing.It is re-sistant to RNase R,has a length of 260 nucleotides,and is predominantly located in the cytoplasm.circCAMSAP2 expression is downregulated during PDCoV replication.Overexpression of circ-CAMSAP2 inhibited PDCoV proliferation,while knockdown promoted it in ST cells.These find-ings suggest that porcine circCAMSAP2 acts as a negative regulator of PDCoV replication and could serve as a potential target for the development of anti-PDCoV therapeutics.
