Establishment and evaluation of RPA-LFD rapid detection method for Campy-lobacter jejuni

VernacularTitle:空肠弯曲菌RPA-LFD快速检测方法的建立及评价
Author: Jingfen YE ¹ ; Shaobi WU ; Shixiong CHEN ; Youci LONG ; Yiwen LIAO ; Xue LUO ; Qi YANG
Author Information

1. 贵州大学动物科学学院,贵州贵阳 550025
Publication Type:Journal Article
Keywords: Campylobacter jejuni; RPA-LFD; hipO gene
From: Chinese Journal of Veterinary Science 2024;44(12):2579-2584
CountryChina
Language:Chinese
Abstract: In order to establish a specific,rapid and convenient method for the detection of Campy-lobacter jejuni(C.jejuni).A set of specific primers and a probe that do not cause false positives were designed with the hipO gene of C.jejuni as the target,and the 5'ends of the downstream primers and probes were labeled with biotin and fluorescein,respectively.C.jejuni-RPA-LFD had no cross-reactivity with Klebsiella pneumoniae,Escherichia coli,Pseudomonas,Bacillus cereus,Pasteurella,Proteus mirabilis,and Salmonella typhimurium,and the optimal reaction system was 37 ℃,25 min,and its sensitivity could reach 3.93×100 copies/μL,and 1 × 102 CFU/mL of C.jejuni contaminated stool samples could be detected in the simulated detection.The C.jejuni-RPA-LFD established in this study has the advantages of good specificity,simplicity,rapidity and high sensitivity,which provides an effective way for the rapid diagnosis of C.jejuni and the con-trol of the spread of C.jejuni at the grassroots level of livestock and poultry farming.