Study on vestibular Schwannoma-derived exosomes inducing ferroptosis in HEI-OC1 cells
10.3969/j.issn.1006-7299.2025.06.012
- VernacularTitle:前庭神经鞘膜瘤源性外泌体诱导HEI-OC1细胞铁死亡的研究
- Author:
Maimaitiming DILIHUMAER
1
;
Yuewen GAO
1
;
Zhaohui WANG
1
;
Zirong HUO
1
;
Tao YANG
1
;
Zhihua ZHANG
1
Author Information
1. 上海交通大学医学院附属第九人民医院耳鼻咽喉头颈外科(上海 200029);上海交通大学医学院耳科学研究所(上海 200029);上海市耳鼻疾病转化医学重点实验室(上海 200029)
- Publication Type:Journal Article
- Keywords:
Vestibular schwannoma;
Extracellular vesicles;
Ferroptosis;
HEI-OC1 cells
- From:
Journal of Audiology and Speech Pathology
2025;33(6):569-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the damage mechanism of extracellular vesicles(EVs)derived from ves-tibular schwannoma(VS)on HEI-OC1 cells and the protective effect of the ferroptosis inhibitor ferrostatin-1(Fer-1).Methods Tumor tissues and clinical data were collected from four patients with stage Ⅱ or Ⅲ VS,classified as grade D according to the AAO-HNS hearing classification.Primary VS cells were extracted,and their conditioned supernatant was collected.EVs were isolated using ultracentrifugation and identified.HEI-OC1 cells were cultured in vitro and divided into three groups:the control group(no treatment),the EVs group(treated with 3000 parti-cles/cell VS-EVs for 24 hours),and the EVs+Fer-1 group(pretreated with 20 μmol/L Fer-1 for 2 hours followed by co-culture with 3000 particles/cell VS-EVs for 24 hours).Cell viability was assessed using the CCK-8 assay,re-active oxygen species(ROS)levels were quantified using the DCFH-DA fluorescent probe,and lipid peroxidation was evaluated using the BODIPY 581/591 C11 probe.Results Compared with the control group,the EVs group showed significantly reduced cell viability(P＜0.001)and increased levels of ROS(P＜0.001)and lipid peroxides(P＜0.001).However,the EVs+Fer-1 group exhibited significantly restored cell viability(P＜0.001)and re-duced levels of ROS and lipid peroxidation(P＜0.001).Conclusion VS-derived EVs disrupts redox homeostasis,promotes intracellular accumulation of lipid peroxides and ROS,and induces ferroptosis in HEI-OC1 cells.Fer-1 significantly alleviates VS-EVs-induced ferroptosis,thereby protecting HEI-OC1 cells from damage.
