The effect of piceatannol on inflammatory response in hepatitis B virus infected rats by regulating Notch signaling pathway
10.16718/j.1009-7708.2025.04.010
- VernacularTitle:白皮杉醇调节Notch信号通路对乙型肝炎病毒感染大鼠炎症反应的影响
- Author:
Qi LIU
1
;
Jing ZHENG
1
;
Lili GAO
1
;
Jiagang ZHANG
1
Author Information
1. 青海省第五人民医院消化内科,西宁 810007
- Publication Type:Journal Article
- Keywords:
piceatannol;
Notch signal;
hepatitis B virus;
infection;
inflammatory reaction;
liver function
- From:
Chinese Journal of Infection and Chemotherapy
2025;25(4):418-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of piceatannol on inflammatory response in hepatitis B virus(HBV)infected rats by regulating Notch signaling pathway.Methods Wistar rats were randomly assigned to control group,model group,low dose piceatannol group,high dose piceatannol group,high dose piceatannol+Jagged1 group to receive injection of HBV antigen solution via tail vein to induce HBV infection model.After intervention with piceatannol and Notch signal activator Jagged1,HBV infection and liver function of rats in each group were tested.ELISA was applied to measure the levels of inflammatory biomarkers in serum and liver tissue.Immunoblotting was applied to detect the expression of Notch signaling pathway related proteins[Notch1,Delta-like ligand 4(DLL4),hairy and enhancer of split 1(Hes1)]in the liver tissues.Results Compared with the control group,the aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels,iNOS and TNF-α levels,Notch1,DLL4,and Hes1 protein expression increased(P＜0.05),IL-10 level decreased(P＜0.05)in model group.AST and ALT,iNOS and TNF-α levels,liver tissue Notch1,DLL4,and Hes1 protein expression decreased,hepatitis B surface antigen(HBsAg)and hepatitis B e antigen(HBeAg)seroconversion rate,IL-10 levels increased(P＜0.05)in the low and high dose piceatannol groups compared with the model group(P＜0.05).High dose piceatannol was associated with stronger beneficial effect on various pathological indicators in HBV infected rats.Jagged1 could reduce the beneficial effect of piceatannol on various pathological indicators in HBV infected rats.Conclusions Piceatannol can reduce the production of proinflammatory factors and increase the production of anti-inflammatory factors by blocking Notch signal transduction,thereby inhibiting inflammation,alleviating liver injury,and improving liver function in HBV-infected rats.
