Study on the effect of Huoxue Jiedu Formula on apoptosis of submaxillary gland cells in mice with Sj?gren syndrome
10.3969/j.issn.1006-2157.2024.12.009
- VernacularTitle:活血解毒方对干燥综合征小鼠颌下腺细胞凋亡的影响研究
- Author:
Dongfeng WANG
1
;
Yuelan ZHU
;
Xiujuan HOU
;
Xinyi GU
;
Hai HU
;
Fangkai LI
Author Information
1. 北京中医药大学第三附属医院 北京 100029
- Publication Type:Journal Article
- Keywords:
Huoxue Jiedu Formula;
Sj?gren syndrome;
submaxillary gland;
cell apoptosis;
mice
- From:
Journal of Beijing University of Traditional Chinese Medicine
2024;47(12):1693-1701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Huoxue Jiedu Formula on the expression of the apoptosis-related factors B-cell lymphoma-2(Bcl-2)and survivin in submaxillary gland cells in mice with Sj?gren syndrome(SS).Methods Using 15 healthy C57BL/6J female mice as the blank control group.Seventy-five of non-obese diabetes mellitus female mice were classified as SS model.They were randomly assigned to the model group,total glucosides of paeony capsule group(TGP group,0.233 g/kg),Huoxue Jiedu Formula low-(15.925 g/kg),middle-(31.850 g/kg),and high-dose(62.335 g/kg)groups,with 15 mice in each group.The blank control and model groups received distilled water by gavage,and the other groups received respective drug doses by gavage once a day for 8 consecutive weeks.After 1,2,4,6,and 8 weeks of gavage,the salivary secretion volume of the mice was measured.The submaxillary gland index was calculated.Pathological changes in the submaxillary gland of mice were observed by hematoxylin-eosin staining.The expression of apoptosis-related factors Bcl-2 and survivin was detected by Western blotting and real-time fluorescence PCR.Results There was statistical significance in salivary secretion volume from aspects of between-group,time-point,and interaction effects(P<0.05).After 8 weeks of treatment,compared to the blank control group,salivary secretion volume,submaxillary gland index,and protein and mRNA expressions of Bcl-2 and survivin in the model group were decreased,while the pathological score grading increased(P<0.05).Compared to the model group,the TGP group and the Huoxue Jiedu Formula low-,medium-,and high-dose groups showed an increase in saliva secretion after 8 weeks of treatment and an increase in submaxillary gland index,Bcl-2,and survivin mRNA expression levels,and a decrease in pathological score levels.The protein expressions of Bcl-2 and survivin in the Huoxue Jiedu Formula medium-and high-dose groups were increased(P<0.05).Compared to the TGP group,salivary secretion volume and submaxillary gland index in the Huoxue Jiedu Formula low-dose group were lower at weeks 4 and 6,while the pathological score grading was higher(P<0.05).The pathological score grading in the Huoxue Jiedu Formula high-dose group was lower.The protein and mRNA expressions of Bcl-2 in the Huoxue Jiedu Formula low-and medium-dose groups were lower(P<0.05).Conclusion Huoxue Jiedu Formula may relieve the submaxillary gland lesions and increase the salivary secretion volume of the submaxillary gland in SS model mice by regulating the expressions of apoptosis-related factors such as Bcl-2 and survivin in the submaxillary gland.
