A comparative study of NQAD and ELSD for the determination of peimine and peiminine in huangshi xiangsheng pills
10.19778/j.chp.2024.06.001
- VernacularTitle:NQAD和ELSD在黄氏响声丸中贝母素甲和贝母素乙含量测定的对比研究
- Author:
Huiyi LIU
1
;
Limin ZUO
;
Xiaodan QIU
;
Xin GUO
;
Xiaofang LIAN
;
Qingying JIA
;
Yongsheng GU
;
Xuanlin LIU
;
Guangzhi SHAN
Author Information
1. 中国医学科学院北京协和医学院医药生物技术研究所,北京 100050
- Publication Type:Journal Article
- Keywords:
high performance liquid chromatography;
nano quantity analyte detector;
evaporative light scattering detector;
huangshi xiangsheng pills;
peimine;
peiminine
- From:
Drug Standards of China
2024;25(6):529-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The study aimed to quantify the contents of peimine and peiminine in huangshi xiangsheng pills using a nano quantity analyte detector(NQAD)and compare the results with the evaporative light scattering detector(ELSD).Methods:Analytical separation was conducted utilizing a CAPCELL PAK MG Ⅲ C18 column(4.6 mm ×250 mm,5 μmi)as the stationary phase.The mobile phase consisted of a mixture of acetonitrile-water-diethylamine(65∶35∶0.03),with a flow rate set at 1.0 mL·min-1 and the column maintained at a temperature of 25℃.Results:NQAD analysis revealed a robust linear correlation between the concentration and the peak area for peimine within a concentration range of 2.450 to 1.470 μg·mL-1,achieving a correlation coefficient of 0.999 3.The limit of detection(LOD)and recovery rate for peimine were determined to be 14.70 ng and 99.50％(n=9),respectively.Similarly,a linear relationship was observed for peiminine between the concentration and the peak area,spanning a range of 3.657 to 1.170 μg·mL-1,with a correlation coefficient of 0.999 1.The LOD and recovery rate for peiminine were 21.94 ng and 100.5％(n=9),respectively.In contrast,ELSD yielded LOD of 24.50 ng for peimine and 36.57 ng for peiminine,with linear range of double logarithmic fitting was 4.900 to 490.0 μg·mL-1 and 7.314 to 585.1 μg·mL-1,respectively.Conclusion:NQAD outperforms ELSD in the quantification of peimine and peiminine,offering superior accuracy,precision,and sensitivity,along with an extended dynamic linear range.By enabling direct content calculation via linear regression,it streamlines the process,bypassing the complex double logarithmic calculations.This approach not only boosts the efficiency of data handling but also substantially simplifies the computational steps,addressing the detection and analysis needs for peimine and peiminine in huangshi xiangsheng pills.
