Effect of Yiqi Huoxue Formula on pulmonary alveolar-capillary barrier function in the rat of acute lung injury induced by lipopolysaccharide
10.3969/j.issn.1006-2157.2024.12.010
- VernacularTitle:益气活血方对脂多糖致急性肺损伤大鼠肺气血屏障功能的影响
- Author:
Li QIN
1
;
Ying XIANG
1
;
Han LI
1
;
Min LI
1
;
Xiaorong QI
1
Author Information
1. 中国人民解放军总医院第六医学中心中医医学部 北京 100853
- Publication Type:Journal Article
- Keywords:
acute lung injury;
Yiqi Huoxue Formula;
pulmonary alveolar-capillary barrier;
tight junction protein;
aquaporin;
rats
- From:
Journal of Beijing University of Traditional Chinese Medicine
2024;47(12):1702-1709
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to reveal the effects of the Yiqi Huoxue Formula(YQHXF)on the function of the pulmonary alveolar-capillary barrier in rats with acute lipopolysaccharide-induced lung injury and to explore the potential mechanism.Methods Sixty adult male SD rats were randomly assigned to the sham operation,model,dexamethasone(5 mg/kg,gavage),Yiqi Huoxue Formula low-dose(3.6 g/kg,gavage),medium-dose(7.2 g/kg,gavage)and high-dose(14.4 g/kg,gavage)groups.The rats in the sham group received intratracheal instillation of normal saline,and those in other groups received intratracheal instillation of lipopolysaccharide(LPS,5 mg/kg)for the modeling of ALI.The rats in the sham and model groups were administered normal saline by gavage,and those in the other groups were administered the corresponding drugs once a day for 7 days.Specimens were collected 12 h after the last administration.Pathological changes in lung tissue were observed under a light microscope.The wet/dry weight ratio of the lung and the total protein concentration in the bronchoalveolar lavage fluid(BALF)were measured.Immunofluorescence staining was used to measure the protein expression of zonula occludens-1(ZO-1).Western blotting was used to measure protein levels of occludin,aquaporin-1(AQP-1),and AQP-5.Results Compared with the sham group,the model group showed an increased histopathological score of the lung tissue,lung wet/dry weight ratio,and protein concentration in BALF(P<0.01).Yiqi Huoxue Formula medium-and high-dose groups were lower than those in the model group(P<0.05,P<0.01).Compared with the sham group,the model group downregulated the expression of ZO-1,occludin,AQP-1,and AQP-5(P<0.01).Yiqi Huoxue Formula medium-and high-dose groups upregulated the protein levels compared with the model group(P<0.01).Conclusion The findings suggest that the Yiqi Huoxue Formula can improve the dysfunction of the pulmonary alveolar-capillary barrier in the rat model of ALI induced by LPS,and its mechanism is related to regulating tight junction protein(ZO-1,occludin)and aquaporin(AQP-1,AQP-5)expression.
