Study on the effect of galangin on bone metabolism by regulating Hippo/Yes-related protein signaling pathway in rats with diabetic osteoporosis
10.3969/j.issn.1006-6187.2025.02.011
- VernacularTitle:高良姜素调节Hippo/Yes相关蛋白信号通路对糖尿病骨质疏松大鼠骨代谢影响的研究
- Author:
Bi HUANG
1
;
Meng HAO
1
;
Xiang OU
1
;
Huige SHAO
1
Author Information
1. 410028 长沙市中心医院内分泌科
- Publication Type:Journal Article
- Keywords:
Galangin;
Hippo/Yes associated protein signaling pathways;
Diabetic osteoporosis;
Bone metabolism
- From:
Chinese Journal of Diabetes
2025;33(2):136-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of galangin(GAL)on bone metabolism by regulating Hippo/Yes associated protein(YAP)signaling pathway in rats with diabetic osteoporosis(DOP).Methods 90 female SD rats were randomly divided into normal control(NC)group,model(Mod)group,low-dose GAL(GAL-L)group,high-dose GAL(GAL-H)group,and high-dose GAL+YAP inhibitor Verteporfin(GAL-H+Verteporfin)group,with 18 rats in each group.Dual energy X-ray was used to detect bone mineral density(BMD).Three-point bending assay was used to evaluate the biomechanics of femur.HE staining was used to test the pathological changes in femoral tissue.ELISA was used to detect serum levels of osteocalcin(OC),alkaline phosphatase(ALP),and tartaric-resistant acid phosphatase(TRAP).Western blot was used to evaluate the expression of YAP protein in femoral tissue.Results Compared with NC group,the BMD,maximum load,elastic modulus,OC,ALP,and YAP protein expression of femur were reduced(P<0.05),and the TRAP was increased in Mod group(P<0.05).Compared with Mod group,BMD,maximum load,elastic modulus,OC,ALP and YAP protein expressions of femur were increased(P<0.05),while TRAP was decreased in GAL-L and GAL-H groups(P<0.05),and the improvement was better in GAL-H group.Compared with GAL-H group,femur BMD,maximum load,elastic modulus,OC,ALP and YAP protein expressions of femur were decreased(P<0.05),while TRAP was increased in GAL-H+Verteporfin group(P<0.05).Conclusions GAL may regulate the Hippo/YAP signaling pathway,increase the expression of YAP,regulate bone metabolism in DOP rats,and inhibit bone resorption.
