Study on the mechanism of Xiongshi Shiwei Wendan decoction pro-moting RCT and treat AS based on network pharmacology,molecular docking and in vitro experiment
10.12092/j.issn.1009-2501.2025.08.003
- VernacularTitle:基于网络药理学、分子对接及体外实验探讨熊氏十味温胆汤促胆固醇逆转运防治动脉粥样硬化的作用机制
- Author:
Xingyu MA
1
;
Xuejiao XIE
;
Chunqiao LI
;
Zheng ZHANG
Author Information
1. 山东中医药高等专科学校,烟台 264199,山东;湖南中医药大学,长沙 410208,湖南
- Publication Type:Journal Article
- Keywords:
Xiongshi Shiwei Wendan decoction;
atherosclerosis;
reverse cholesterol transport;
AB-CA1;
miR-33
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(8):1026-1036
- CountryChina
- Language:Chinese
-
Abstract:
AIM:Xiongshi Shiwei Wendan decoc-tion(SWD)comes from Xiong Jibai,a master of tra-ditional Chinese medicine,and has been widely used in the treatment of AS.ABCA1 is an important pathway for macrophages to export cholesterol and plays a protective role in the occurrence and development of AS.The purpose of this study was to study the effects of SWD on ABCA1 expression and cholesterol efflux through network pharmacol-ogy,molecular docking and in vitro experiments,and explore the pathway mechanism of promoting reverse cholesterol transport(RCT).METHODS:The active components of SWD drugs were screened by TCMSP and HERB databases,RCT targets were pre-dicted,the component-target network map was constructed,the PPI network was constructed and the GO and KEGG pathways were enriched and ana-lyzed by STRING database,and the key active com-ponents of SWD were selected for molecular dock-ing with ABCA1 protein and miR-33 by AutoDockVi-na.In vitro,RAW264.7 was used to establish foam cell model,oil red O staining,NBD-cholesterol staining and lentivirus overexpression cell miRNA-33 were used to study the effect of SWD on lipid accumulation and cholesterol outflow rate of RAW264.7 cells.Western blotting was used to de-tect the expression of ABCA1.RESULTS:According to network pharmacology,336 active components of SWD,267 targets of RCT and 46 targets of inter-section of RCT and SWD were obtained,which in-volved multiple signal pathways such as lipid and atherosclerosis.Molecular docking showed that the main active components had stable conforma-tion with ABCA1 and miR-33.In vitro experiment,it was found that the lipid content was significantly decreased(P＜0.01),the cholesterol outflow rate was significantly increased(P＜0.01)and the expres-sion of ABCA1 protein was up-regulated in SWD group(P＜0.01),but the expression of ABCA1 in miR-33 overexpression group was significantly de-creased(P＜0.01).CONCLUSION:SWD has the char-acteristics of multi-components and multi-targets,which can promote RCT and treat AS through miR-NA-33-ABCA1 pathway.
