Research on water-soluble fibre regulating intestinal flora and improving insulin resistance in rats
10.3969/j.issn.1006-6187.2025.02.012
- VernacularTitle:水溶性纤维素调控肠道菌群改善大鼠胰岛素抵抗的研究
- Author:
Hong LONG
1
;
Shibin ZHANG
1
;
Tingyang QIN
1
Author Information
1. 563006 遵义医科大学实验动物中心
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Branch chain amino acids;
Water-soluble fibre;
Intestinal flora
- From:
Chinese Journal of Diabetes
2025;33(2):142-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between water-soluble fibre and insulin resistance(IR)through regulating intestinal flora.Methods 24 male SD rats were randomly divided into normal control(NC)group,high-fat diet(HFD)group,and mixed 10%water-soluble fibre(F-HFD)group,with eight rats in each group.Body weight,blood glucose,serum branch chain amino acids(BCAAs)and FIns were measured.Glucose tolerance test and insulin tolerance test were performed.ISI and assessment-insulin resistance(HOMA-IR)was calculated.16SrDNA gene amplification and sequencing were performed to determine the composition of gut microorganisms in the intestinal contents of the rats.Results The FPG,HOMA-IR,and BCAAs were sequentially increased.FIns was higher in HFD group than in NC and F-HFD groups(P<0.05),ISI was lower in HFD group than in NC and F-HFD groups(P<0.05).The relative abundance of Prevotella and Bacteroides in feces was significantly higher(P<0.05)and Roseburia was significantly lower(P<0.05)in the HFD group compared with the NC group.The relative abundance of Prevotella was decreased in the F-HFD group compared with the HFD group(P<0.05).Correlation analysis showed that BCAAs were positively correlated with IR,glucose tolerance and Prevotella(P<0.05).Conclusions Water-soluble fibre may reduce the production of BCAAs by down-regulating the abundance of intestinal flora Prevotella in rats on a high-fat diet,leading to IR reduction.
