Influence of surface structure of 3D-printed silk fibroin-based scaffolds on bronchial epithelial cells growth in vitro
10.3969/j.issn.1672-8467.2025.04.001
- VernacularTitle:3D打印丝素蛋白支架结构对支气管上皮细胞体外生长的影响
- Author:
Zhong-chun CHEN
1
;
Nong-ping ZHONG
;
Tao DONG
;
Zheng-zhong SHAO
;
Xia ZHAO
Author Information
1. 复旦大学附属华山医院耳鼻咽喉头颈外科 上海 200040
- Publication Type:Journal Article
- Keywords:
silk fibroin(SF);
3D printing;
epithelial cell;
bronchus;
surface morphology;
porosity
- From:
Fudan University Journal of Medical Sciences
2025;52(4):475-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the structure of 3D-printed silk fibroin/hydroxypropyl methylcellulose(SF/HPMC)scaffolds on the growth of tracheal epithelial cells in vitro.Methods Six types of SF/HPMC scaffolds with different surface topography,pore size,and porosity were fabricated using a 3D printer by adjusting the concentration of SF/HPMC solutions and printing parameters,combined with freeze-drying.Normal human bronchial epithelial cell lines BEAS-2B were cultured on these scaffolds for 7 days.The cell proliferation was detected by CCK-8 assay and live/dead cell staining,and the cell morphology was observed by scanning electron microscopy(SEM).Results The porosity of 20%(weight percentage)SF/HPMC scaffolds with rough surface and smooth surface were 70.5%±2.0%and 65.5%±6.1%,respectively,and the porosity of 30%(weight percentage)SF/HPMC scaffolds with rough surface and smooth surface were 63.9%±2.1%and 59.6%±2.1%,respectively.The two pore sizes of the rough-surfaced 20%SF/HPMC scaffolds were(443.9±104.1)μm and(681.1±115.1)μm.BEAS-2B cells spread better on the rough-surfaced scaffolds,and their proliferation was higher on scaffolds with higher porosity and smaller pore sizes compared to those with lower porosity and larger pore sizes.Conclusion The 3D-printed SF/HPMC scaffolds are suitable for bronchial epithelial cell growth.Scaffolds with rough surfaces,higher porosity,and appropriate pore sizes might facilitate BEAS-2B cell growth.
