Interference of Chylomicronemia in Serum Lithium Detection by the Phosphatase Method and Optimization Strategies
10.3969/j.issn.1671-7414.2025.04.036
- VernacularTitle:乳糜血对磷酸酶法检测血清锂浓度的干扰及应对处理措施
- Author:
Sihai LING
1
;
Shan HE
1
;
Meiping WEI
1
;
Jing HE
1
;
Xiaomei QI
1
;
Chengeng LIU
1
Author Information
1. 首都医科大学附属北京安定医院检验科,北京 100088
- Publication Type:Journal Article
- Keywords:
chylomicronemia;
serum lithium;
phosphatase method;
high-speed centrifugation;
physiological saline dilution
- From:
Journal of Modern Laboratory Medicine
2025;40(4):199-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interference of varying degrees of lipemia on serum lithium(Li)measurement using the phosphatase method and explore effective mitigation strategies.Methods A pooled sample of severe lipemic serum without lithium was collected and concentrated to obtain lipemic serum concentrate.A pooled serum sample from patients with normal lipid levels taking lithium carbonate was used for triglyceride(TG)interference experiments.Additionally,28 serum samples from patients not taking lithium carbonate were collected.Based on the results of TG interference experiments,the maximum TG concentration that did not interfere with serum lithium measurement was determined as the target concentration for dilution,and the corresponding dilution factor was calculated.Lithium solution was added to each sample to determine the theoretical lithium concentration.Samples were divided into three groups and analyzed using direct detection,physiological saline dilution,and high-speed centrifugation(13 000 r/min,10 min).The results obtained from different methods were compared.Results Lipemic serum with TG concentrations>4.77mmol/L interfered with lithium measurement by the phosphatase method.The physiological saline dilution method showed the largest deviation(t=10.87,P<0.000 1)and significant differences from theoretical values,making it unsuitable for accurate measurement.In contrast,the high-speed centrifugation method provided results closest to the theoretical values(t=2.97,P=0.036 9)with higher accuracy.Although the direct detection method was highly correlated with the high-speed centrifugation method(r=0.976 5,P<0.000 1),with a significant mean difference remained(t=5.37,P<0.000 1).Conclusion For lipemic serum samples with TG concentrations>4.77mmol/L,the physiological saline dilution method should be avoided due to its inaccuracy.High-speed centrifugation effectively removes lipemic interference,yielding results closer to theoretical values,and is recommended as the optimized method for serum lithium measurement in lipemic samples.
