Effects of uncinine on lipid metabolism in hepatocytes deficient in glucose-6-phosphatase
10.13699/j.cnki.1001-6821.2024.24.016
- VernacularTitle:钩藤碱对葡萄糖-6-磷酸酶缺乏的肝细胞脂质代谢的研究
- Author:
Ying-lian ZHANG
1
;
Ya-ji WU
1
;
Yan-fang LI
1
Author Information
1. 厦门大学附属妇女儿童医院/厦门市妇幼保健院新生儿疾病筛查实验室,福建厦门 361001
- Publication Type:Journal Article
- Keywords:
uncinine;
glucose-6-phosphatase;
abnormal liver lipid metabolism;
autophagy;
adenosine monophosphate activated protein kinase/mammalian target of rapamycin
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(24):3601-3605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of uncinine on abnormal lipid metabolism in hepatocytes caused by glucose-6-phosphatase(G-6-Pase)deficiency and its mechanism.Methods Mouse hepatocytes AML12 were randomly divided into blank group(normal culture),si-NC group(transfected with si-NC plasmid),si-G-6-Pase group(transfected with si-G-6-Pase plasmid),si-G-6-Pase+DMSO group[transfected with si-G-6-Pase+solvent dimethyl sulfoxide(DMSO)],si-G-6-Pase+Uncarine group(transfected with si-G-6-Pase+80.0 μmol·L-1 uncarine)and si-G-6-Pase+Uncarine+3-MA group[transfected with si-G-6-Pase+80.0 μmol·L-1 uncarine+5.0 mmol·L-1 3-methyladenine(3-MA)].The kit was used to detect triglyceride(TG)and total cholesterol(TC),Western blot assay was used to detect protein expression.Results The levels of TG in blank group,si-NC group,si-G-6-Pase group,si-G-6-Pase+DMSO group and si-G-6-Pase+Uncarine group were(0.13±0.01),(0.13±0.01),(0.20±0.02),(0.21±0.02)and(0.17±0.01)μ,mol·L-1·mg protein-1;the TC levels were(0.08±0.01),(0.07±0.01),(0.15±0.01),(0.16±0.01)and(0.10±0.01)μmol·L-1·mg protein-1;the Beclin 1 protein relative expression were 0.79±0.06,0.80±0.07,0.42±0.07,0.41±0.03 and 0.70±0.05;the relative expression of nucleus transcription factor EB(TFEB)protein were 0.47±0.05,0.45±0.04,0.19±0.03,0.20±0.03 and 0.59±0.06;the phosphorylated-adenosine monophosphate activated protein kinase(p-AMPK)protein relative expression were 0.91±0.13,0.93±0.07,0.54±0.07,0.55±0.06 and 0.70±0.09,respectively.The above indicators:si-G-6-Pase group were compared with si-NC group,si-G-6-Pase+Uncarine group were compared with si-G-6-Pase+DMSO group,the differences were statistically significant(all P＜0.05).The levels of TG in si-G-6-Pase+Uncarine group and si-G-6-Pase+Uncarine+3-MA group were(0.19±0.03)and(0.12±0.01)μmol·L-1·mg protein-1;the TC levels were(0.10±0.01)and(0.15±0.01)μmol·L-1·mg protein-1,respectively,the differences were statistically significant(all P＜0.05).Conclusion Uncarine may improve the abnormal liver lipid metabolism caused by G-6-Pase deficiency by enhancing autophagy.
