Research of Esculetin inhibiting the malignant biological behavior of cervical cancer cells
10.13699/j.cnki.1001-6821.2024.24.012
- VernacularTitle:秦皮乙素抑制宫颈癌细胞恶性生物学行为的研究
- Author:
Jin-yan ZHONG
1
;
Lin-lin XU
1
;
Xin LU
1
;
Xue-meng RAN
1
Author Information
1. 青岛大学附属青岛市海慈医院/青岛市中医医院妇科,山东青岛 266033
- Publication Type:Journal Article
- Keywords:
Esculetin;
cervical carcinoma;
micro-ribonucleic acid-617;
small ubiquitin-like modifier activating enzyme subunit 1;
malignant behavior;
invasion;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2024;40(24):3579-3584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Esculetin inhibiting the malignant biological behavior of cervical cancer cells by targeting small ubiquitin-like modifier activating enzyme subunit 1(SAE1)via microRNA-617(miR-617).Methods SiHa cells were divided into SiHa group(normal culture),Esculetin group(4 mmol·L-1Esculetin treatment),Esculetin+NC inhibitor+sh-NC group(4 mmol·L-1 Esculetin+miR-617 inhibition negative control+SAE1 inhibition negative control),Esculetin+miR-617 inhibitor+sh-NC group(4 mmol·L-1 Esculetin+miR-617 inhibition+SAE1 inhibition negative control)and Esculetin+miR-617 inhibitor+sh-SAE1 group(4 mmol·L-1 Esculetin+miR-617 inhibition+SAE1 inhibition).The expression of miR-617 and SAE1 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Cell invasion and apoptosis were detected by Transwell and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL).Results The relative expression levels of miR-617 in SiHa group,Esculetin group,Esculetin+NC inhibitor+sh-NC group,Esculetin+miR-617 inhibitor+sh-NC group and Esculetin+miR-617 inhibitor+sh-SAE1 group were 1.00±0.16,1.93±0.26,2.29±0.31,1.18±0.16 and 1.59±0.23,respectively;the relative expression levels of SAE1 mRNA were 1.00±0.13,0.67±0.08,0.37±0.06,0.74±0.14 and 0.41±0.08,respectively;the number of invasive cells in SiHa group,Esculetin+NC inhibitor+sh-NC group,Esculetin+miR-617 inhibitor+sh-NC group and Esculetin+miR-617 inhibitor+sh-SAE1 group were 102.79±12.37,37.16±5.84,106.36±14.20 and 61.25±11.06,respectively;the apoptosis rates were(11.50±2.23)％,(43.66±6.82)％,(15.35±3.92)％and(38.16±4.67)％,respectively;the above indicators in Esculetin group were statistically significant when compared with SiHa group(all P＜0.05);the above indicators in Esculetin+NC inhibitor+sh-NC group were statistically significant when compared with Esculetin group(all P＜0.001);the above indicators in Esculetin+miR-617 inhibitor+sh-NC group the above indicators were statistically significant when compared with Esculetin+NC inhibitor+sh-NC group(all P＜0.001);Esculetin+miR-617 inhibitor+sh-SAE1 group the above indicators were statistically significant when compared with Esculetin+miR-617 inhibitor+sh-NC group(all P＜0.01).Conclusion Esculetin can inhibit the invasion of cervical cancer cells and promote apoptosis by regulating the miR-6177/SAE1 axis.
